Abstract
A novel single mode tapered optical fibre loop biochemical sensor based on fluorescence spectroscopy has been developed. The fundamental fibre mode propagating through the tapered portion of the waveguide has evanescent fields which penetrate into the aqueous environment where the biochemical recognition event occurs. The model measurands were conjugated with a fluorescent dye, fluorescein isothiocyanate. When excited by the input laser light from the near end of the taper, generated fluorescence is coupled into the guided mode ofthe fibre and collected at the far end of the taper. Several radioactive and fluorescent quantification methods have been explored to determine the density of available binding sites immobilised on the fibre, and thus the ultimate sensitivity of the device. A generic avidin-biotin system has been tested as a model immunological diagnostic system. The high sensitivity of a single mode tapered loop device combined with a simple immobilisation method provides a powerful tool for performing immunoassays.
Original language | English |
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Pages (from-to) | 484-494 |
Number of pages | 11 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 2131 |
DOIs | |
State | Published - 28 Jul 1994 |
Externally published | Yes |
Event | Biomedical Fiber Optic Instrumentation 1994 - Los Angeles, United States Duration: 23 Jan 1994 → 29 Jan 1994 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering