The single mode tapered optical fibre loop immunosensor: II. Assay of anti-cholera toxin immunoglobulins

R. S. Marks, Z. M. Hale, M. M. Levine, C. R. Lowe, F. P. Payne

Research output: Contribution to journalConference articlepeer-review

7 Scopus citations


An evanescent wave immunoassay for cholera antitoxin immunoglobulins was performed using a single mode tapered optical fibre loop sensor. The transducer was silanised with 3-glycidoxypropyltrimethoxysilane and chemically modified to link covalently either cholera toxin B subunit or a synthetic peptide derived from it, CTP3. The sensor was exposed to semI fluids, obtained from human volunteers having been exposed to live virulent Vibrio cholerae 01 and shown to produce rice-water stools. Other toxins of interest, such as Clostridium botulinum toxin A, have been tested on similar systems [19]. The bound unlabelled immunoglobulins were then exposed to a mixture of FJTC-anti-IgG and TRITC-anti-IgA, without requirement for a separation step. The emanating fluorescent emissions of fluorescein and rhodamine, excited by the input laser light, were coupled back into the guided mode of the tapered fibre, and used to determine the concentrations of the complementary antigens.

Original languageEnglish
Pages (from-to)495-503
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 28 Jul 1994
Externally publishedYes
EventBiomedical Fiber Optic Instrumentation 1994 - Los Angeles, United States
Duration: 23 Jan 199429 Jan 1994

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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