The thermoacidophilic archaeon Sulfolobus acidocaldarius contains an unsually short, highly reduced dolichyl phosphate

Ziqiang Guan, Benjamin H. Meyer, Sonja Verena Albers, Jerry Eichler

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Polyprenoids, polymers containing varied numbers of isoprene subunits, serve numerous roles in biology. In Eukarya, dolichyl phosphate, a phosphorylated polyprenol bearing a saturated α-end isoprene subunit, serves as the glycan carrier during N-glycosylation, namely that post-translational modification whereby glycans are covalently linked to select asparagine residues of a target protein. As in Eukarya, N-glycosylation in Archaea also relies on phosphorylated dolichol. In this report, LC-ESI/MS/MS was employed to identify a novel dolichyl phosphate (DolP) in the thermoacidophilic archaeon, Sulfolobus acidocaldarius. The unusually short S. acidocaldarius DolP presents a degree of saturation not previously reported. S. acidocaldarius DolP contains not only the saturated α- and ω-end isoprene subunits observed in other archaeal DolPs, but also up to five saturated intra-chain isoprene subunits. The corresponding dolichol and hexose-charged DolP species were also detected. The results of the present study offer valuable information on the biogenesis and potential properties of this unique DolP. Furthermore, elucidation of the mechanism of α-isoprene unit reduction in S. acidocaldarius dolichol may facilitate the identification of the alternative, as yet unknown polyprenol reductase in Eukarya.

Original languageEnglish
Pages (from-to)607-616
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1811
Issue number10
DOIs
StatePublished - 1 Oct 2011

Keywords

  • Archaea
  • Dolichol
  • Electrospray ionization mass spectrometry
  • Polyprenol
  • Polyprenol reductase
  • Sulfolobus acidocaldarius

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