The timed-pregnant baboon animal model can be used for determining the role of soluble vascular endothelial growth factor receptors 1 and 2 during development

Joaquin Santolaya-Forgas, Samuel Edwin, Katie Zeiter, Adam Pitt, Beth Pineles, Adi L. Tarca, Jimmy Espinoza, Juan Pedro Kusanovic, Offer Erez, Roman Wolf, Roberto Romero

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: During pregnancy, mechanisms that allow for regulation of continuous fetal and placental vasculogenesis with prevention of maternal neo-vascularization remain elusive. The vascular endothelial growth factor (VEGF) biological system has a key role during vasculogenesis. The aims of this study were to validate a bioassay for soluble vascular endothelial growth factor receptors 1 and 2 (sVEGFR-1 and sVEGFR-2) in baboon plasma and to determine the maternal and fetal plasma concentration of these receptors at the end of the baboon pregnancy. Methods: Maternal peripheral blood samples were obtained from eight baboons (Papio anubis) prior to elective cesarean section and from the umbilical cord after the fetuses were delivered. Spike and recovery experiments at various concentrations in pooled baboon maternal plasma were used to validate a human quantitative sandwich immunoassay for sVEGFR-1 and -2. Concentrations of sVEGFR-1 and -2 were then determined in maternal and fetal plasma samples. Results: No significant correlations were observed between sVEGFR-1 or -2 concentrations in maternal and fetal circulations. The concentration of sVEGFR-1 was at least 30 times greater and that of sVEGFR-2 approximately two times greater, in maternal than in cord plasma (both P < 0.01). Conclusion: These findings suggest that baboons can be used to study the regulation of vasculogenesis during pregnancy.

Original languageEnglish
Pages (from-to)370-374
Number of pages5
JournalJournal of Medical Primatology
Volume36
Issue number6
DOIs
StatePublished - 1 Dec 2007
Externally publishedYes

Keywords

  • Angiogenesis
  • FLK1
  • FLT1
  • Placental vascularization
  • VEGF-receptors
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Veterinary (all)

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