The underappreciated diversity of bile acid modifications

Ipsita Mohanty; Helena Mannochio-Russo; Joshua V. Schweer; Yasin El Abiead; Wout Bittremieux; Shipei Xing; Robin Schmid; Simone Zuffa; Felipe Vasquez; Valentina B. Muti; Jasmine Zemlin; Omar E. Tovar-Herrera; Sarah Moraïs; Dhimant Desai; Shantu Amin; Imhoi Koo; Christoph W. Turck; Itzhak Mizrahi; Penny M. Kris-Etherton; Kristina S. Petersen; Jennifer A. Fleming; Tao Huan; Andrew D. Patterson; Dionicio Siegel; Lee R. Hagey; Mingxun Wang; Allegra T. Aron; Pieter C. Dorrestein

doi:10.1016/j.cell.2024.02.019

The underappreciated diversity of bile acid modifications

Ipsita Mohanty, Helena Mannochio-Russo, Joshua V. Schweer, Yasin El Abiead, Wout Bittremieux, Shipei Xing, Robin Schmid, Simone Zuffa, Felipe Vasquez, Valentina B. Muti, Jasmine Zemlin, Omar E. Tovar-Herrera, Sarah Moraïs, Dhimant Desai, Shantu Amin, Imhoi Koo, Christoph W. Turck, Itzhak Mizrahi, Penny M. Kris-Etherton, Kristina S. PetersenJennifer A. Fleming, Tao Huan, Andrew D. Patterson, Dionicio Siegel, Lee R. Hagey, Mingxun Wang, Allegra T. Aron, Pieter C. Dorrestein

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

Original language	English
Pages (from-to)	1801-1818.e20
Journal	Cell
Volume	187
Issue number	7
DOIs	https://doi.org/10.1016/j.cell.2024.02.019
State	Published - 28 Mar 2024

Keywords

GABA
MassQL
agmatine
bile acids
diet
fastMASST
microbial
polyamines
putrescine
spectral resource

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2024.02.019

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Mohanty, I., Mannochio-Russo, H., Schweer, J. V., El Abiead, Y., Bittremieux, W., Xing, S., Schmid, R., Zuffa, S., Vasquez, F., Muti, V. B., Zemlin, J., Tovar-Herrera, O. E., Moraïs, S., Desai, D., Amin, S., Koo, I., Turck, C. W., Mizrahi, I., Kris-Etherton, P. M., ... Dorrestein, P. C. (2024). The underappreciated diversity of bile acid modifications. Cell, 187(7), 1801-1818.e20. https://doi.org/10.1016/j.cell.2024.02.019

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title = "The underappreciated diversity of bile acid modifications",

abstract = "The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.",

keywords = "GABA, MassQL, agmatine, bile acids, diet, fastMASST, microbial, polyamines, putrescine, spectral resource",

author = "Ipsita Mohanty and Helena Mannochio-Russo and Schweer, {Joshua V.} and {El Abiead}, Yasin and Wout Bittremieux and Shipei Xing and Robin Schmid and Simone Zuffa and Felipe Vasquez and Muti, {Valentina B.} and Jasmine Zemlin and Tovar-Herrera, {Omar E.} and Sarah Mora{\"i}s and Dhimant Desai and Shantu Amin and Imhoi Koo and Turck, {Christoph W.} and Itzhak Mizrahi and Kris-Etherton, {Penny M.} and Petersen, {Kristina S.} and Fleming, {Jennifer A.} and Tao Huan and Patterson, {Andrew D.} and Dionicio Siegel and Hagey, {Lee R.} and Mingxun Wang and Aron, {Allegra T.} and Dorrestein, {Pieter C.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = mar,

day = "28",

doi = "10.1016/j.cell.2024.02.019",

language = "English",

volume = "187",

pages = "1801--1818.e20",

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Mohanty, I, Mannochio-Russo, H, Schweer, JV, El Abiead, Y, Bittremieux, W, Xing, S, Schmid, R, Zuffa, S, Vasquez, F, Muti, VB, Zemlin, J, Tovar-Herrera, OE, Moraïs, S, Desai, D, Amin, S, Koo, I, Turck, CW, Mizrahi, I, Kris-Etherton, PM, Petersen, KS, Fleming, JA, Huan, T, Patterson, AD, Siegel, D, Hagey, LR, Wang, M, Aron, AT & Dorrestein, PC 2024, 'The underappreciated diversity of bile acid modifications', Cell, vol. 187, no. 7, pp. 1801-1818.e20. https://doi.org/10.1016/j.cell.2024.02.019

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T1 - The underappreciated diversity of bile acid modifications

AU - Mohanty, Ipsita

AU - Mannochio-Russo, Helena

AU - Schweer, Joshua V.

AU - El Abiead, Yasin

AU - Bittremieux, Wout

AU - Xing, Shipei

AU - Schmid, Robin

AU - Zuffa, Simone

AU - Vasquez, Felipe

AU - Muti, Valentina B.

AU - Zemlin, Jasmine

AU - Tovar-Herrera, Omar E.

AU - Moraïs, Sarah

AU - Desai, Dhimant

AU - Amin, Shantu

AU - Koo, Imhoi

AU - Turck, Christoph W.

AU - Mizrahi, Itzhak

AU - Kris-Etherton, Penny M.

AU - Petersen, Kristina S.

AU - Fleming, Jennifer A.

AU - Huan, Tao

AU - Patterson, Andrew D.

AU - Siegel, Dionicio

AU - Hagey, Lee R.

AU - Wang, Mingxun

AU - Aron, Allegra T.

AU - Dorrestein, Pieter C.

PY - 2024/3/28

Y1 - 2024/3/28

N2 - The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

AB - The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

KW - GABA

KW - MassQL

KW - agmatine

KW - bile acids

KW - diet

KW - fastMASST

KW - microbial

KW - polyamines

KW - putrescine

KW - spectral resource

UR - http://www.scopus.com/inward/record.url?scp=85188521465&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2024.02.019

DO - 10.1016/j.cell.2024.02.019

M3 - Article

C2 - 38471500

AN - SCOPUS:85188521465

SN - 0092-8674

VL - 187

SP - 1801-1818.e20

JO - Cell

JF - Cell

IS - 7

ER -

The underappreciated diversity of bile acid modifications

Abstract

Keywords

ASJC Scopus subject areas

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