The use of FTIR microscopy for evaluation of herpes viruses infection development kinetics

Vitaly Erukhimovitch, Igor Mukmanov, Marina Talyshinsky, Yelena Souprun, Mahmoud Huleihel

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    The kinetics of Herpes simplex infection development was studied using an FTIR microscopy (FTIR-M) method. The family of herpes viruses includes several members like H. simplex types I and II (HSV I, II), Varicella zoster (VZV) viruses which are involved in various human and animal infections of different parts of the body. In our previous study, we found significant spectral differences between normal uninfected cells in cultures and cells infected with herpes viruses at early stages of the infection. In the present study, cells in cultures were infected with either HSV-I or VZV and at various times post-infection they were examined either by optical microscopy or by advanced FTIR-M. Spectroscopic measurements show a consistent decrease in the intensity of the carbohydrate peak in correlation with the viral infection development, observed by optical microscopy. This decrease in cellular carbohydrate level was used as indicator for herpes viruses infection kinetics. This parameter could be used as a basis for applying a spectroscopic method for the evaluation of herpes virus infection development. Our results show also that the development kinetics of viral infection has an exponential character for these viruses.

    Original languageEnglish
    Pages (from-to)2355-2361
    Number of pages7
    JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
    Volume60
    Issue number10
    DOIs
    StatePublished - 1 Aug 2004

    Keywords

    • FTIR microscopy
    • Herpes viruses
    • Infection development kinetics
    • Spectroscopy
    • Varicella zoster virus

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Atomic and Molecular Physics, and Optics
    • Instrumentation
    • Spectroscopy

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