TY - JOUR
T1 - The usefulness of an inflammation meter to detect the presence of infection/inflammation in elderly patients
AU - Rotstein, Rivka
AU - Zeltser, David
AU - Shapira, Itzhak
AU - Avitzour, Daniel
AU - Fusman, Renato
AU - Dvolatzki, Tzvi
AU - Loewenstein, Anat
AU - Aronson, Moshe
AU - Bornstein, Nathan
AU - Arber, Nadir
AU - Berliner, Shlomo
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Background. Medical personnel who work in small geriatric institutions most frequently do not have access to realtime laboratory facilities. Methods. In order to present a new method to determine the presence of an inflammatory response and for the assessment of its intensity, 118 patients aged 77 ± 6 years with various bacterial infections were evaluated as well as 129 elderly individuals with various stressful conditions but no acute infections who served as controls. The leukocyte and erythrocyte adhesiveness/aggregation tests were performed by using a simple slide test and image analysis. The availability of the CD11b/CD18 and CD62L antigen on the leukocytes' surface was measured by whole blood flow cytometry, and the quantitative C-reactive protein by using laser nephelometry and specific antihuman C-reactive protein antibodies. Results. A significant difference was noted between patients and controls for all variables obtained by the slide test and image analysis. In addition, a highly significant correlation was noted between the number of leukocytes counted on the slides and white blood cell count, between the leukocyte adhesiveness/aggregation test and quantitative C-reactive protein, and between the degree of erythrocyte adhesiveness/aggregation and either the Westergren sedimentation or fibrinogen concentration. Conclusions. By using our low-cost and real-time slide test, any medical or paramedical personnel can get relevant information regarding the presence of an acute phase response at the point of care.
AB - Background. Medical personnel who work in small geriatric institutions most frequently do not have access to realtime laboratory facilities. Methods. In order to present a new method to determine the presence of an inflammatory response and for the assessment of its intensity, 118 patients aged 77 ± 6 years with various bacterial infections were evaluated as well as 129 elderly individuals with various stressful conditions but no acute infections who served as controls. The leukocyte and erythrocyte adhesiveness/aggregation tests were performed by using a simple slide test and image analysis. The availability of the CD11b/CD18 and CD62L antigen on the leukocytes' surface was measured by whole blood flow cytometry, and the quantitative C-reactive protein by using laser nephelometry and specific antihuman C-reactive protein antibodies. Results. A significant difference was noted between patients and controls for all variables obtained by the slide test and image analysis. In addition, a highly significant correlation was noted between the number of leukocytes counted on the slides and white blood cell count, between the leukocyte adhesiveness/aggregation test and quantitative C-reactive protein, and between the degree of erythrocyte adhesiveness/aggregation and either the Westergren sedimentation or fibrinogen concentration. Conclusions. By using our low-cost and real-time slide test, any medical or paramedical personnel can get relevant information regarding the presence of an acute phase response at the point of care.
UR - http://www.scopus.com/inward/record.url?scp=0036182713&partnerID=8YFLogxK
U2 - 10.1093/gerona/57.2.M122
DO - 10.1093/gerona/57.2.M122
M3 - Article
C2 - 11818432
AN - SCOPUS:0036182713
SN - 1079-5006
VL - 57
SP - M122-M127
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 2
ER -