Theoretical analysis of three-dimensional imaging and recognition of micro-organisms with a single-exposure on-line holographic microscope

Adrian Stern, Bahram Javidi

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Single-exposure on-line (SEOL) digital holography is a recently proposed technique for monitoring, visualization, and recognition of three-dimensional (3D) objects. In contrast to traditional multiexposure on-line digital holography, it uses only one exposure, which makes it particularly suitable for imaging and recognizing moving micro-organisms. However, the cost of using only one exposure is the superposition of a conjugate image on the desired reconstructed image. The influence of the conjugate image on the visualization and recognition performance is investigated. The conditions for which the cross-talk noise induced by the conjugate image is negligible are derived. It is demonstrated that with conditions common in imaging of microscopic 3D biological objects, SEOL digital holography is highly tolerant of cross-talk noise induced by the conjugate image.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume24
Issue number1
DOIs
StatePublished - 1 Jan 2007

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

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