Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging

Joseph Rosen, Nisan Siegel, Gary Brooker

Research output: Contribution to journalArticlepeer-review

142 Scopus citations

Abstract

Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.

Original languageEnglish
Pages (from-to)26249-26268
Number of pages20
JournalOptics Express
Volume19
Issue number27
DOIs
StatePublished - 19 Dec 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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