Thiamine deficiency in cardiac cells in culture

Avraham Zangen, Asher Shainberg

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Rat heart cells in culture were found to be a unique model for studying biochemical and pharmacological aspects of thiamine deficiency. When thiamine was excluded from the growth medium, the following effects were observed: (1) Morphological examination did not show any difference between control and thiamine deprived cells during the first 10 days. However, after 10-11 days spontaneous contractions ceased, accompanied by initiation of cell degeneration; (2) Intensive degeneration and cell death were observed after 14-16 days. (3) Thiamine pyrophosphate (TPP) concentration in thiamine-deprived cells was decreased gradually, with an elimination half life of 4-5 days. (4) [3H]deoxyglucose uptake by the cells was increased, even after 1 day of thiamine deprivation. (5) ATP level decreased after 8 days and reached 50% of control cells after 10 days. (6) In thiamine-deprived cells, thiamine addition caused a 60% rise in contraction amplitude but contraction rare was not altered significantly. (7) All these effects were reversible if thiamine was supplied before the initiation of the degeneration processes.

Original languageEnglish
Pages (from-to)575-582
Number of pages8
JournalBiochemical Pharmacology
Volume54
Issue number5
DOIs
StatePublished - 1 Sep 1997
Externally publishedYes

Keywords

  • ATP
  • Contraction amplitude
  • Glucose uptake
  • Rat cardiomyocytes
  • Thiamine

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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