TY - JOUR
T1 - Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence
AU - Maurice, Leanne
AU - Bilenca, Alberto
N1 - Funding Information:
The authors thank Prof. Angel Porgador and Dr. Orly Gershoni-Yahalom of Ben-Gurion University of the Negev for the valuable assistance in the preparation of the biological samples. A.B. acknowledges the support of the Israel Science Foundation (grant no. 1599/12).
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines, including material sciences, physics, and biology, but often shows anisotropic three-dimensional spatial localization precision, which restricts the tracking precision, and/or a limited number of particles that can be tracked simultaneously over extended volumes. Here we developed an interferometric, three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted, high-aperture-angle, fluorescence wavefronts in a greatly simplified, free-running, triangle interferometer that enables tracking of multiple particles at the same time with <10-nm spatial localization precision in all three dimensions over extended volumes (~35 × 35 × 2 μm3) at video rate (25 Hz). We applied our method to characterize the microenvironment of living cells and up to ~40 μm deep in soft materials.
AB - Single particle tracking in three dimensions is an indispensable tool for studying dynamic processes in various disciplines, including material sciences, physics, and biology, but often shows anisotropic three-dimensional spatial localization precision, which restricts the tracking precision, and/or a limited number of particles that can be tracked simultaneously over extended volumes. Here we developed an interferometric, three-dimensional fluorescence single particle tracking method based on conventional widefield excitation and temporal phase-shift interference of the emitted, high-aperture-angle, fluorescence wavefronts in a greatly simplified, free-running, triangle interferometer that enables tracking of multiple particles at the same time with <10-nm spatial localization precision in all three dimensions over extended volumes (~35 × 35 × 2 μm3) at video rate (25 Hz). We applied our method to characterize the microenvironment of living cells and up to ~40 μm deep in soft materials.
UR - http://www.scopus.com/inward/record.url?scp=85149593257&partnerID=8YFLogxK
U2 - 10.1038/s41377-023-01085-7
DO - 10.1038/s41377-023-01085-7
M3 - Article
C2 - 36864021
AN - SCOPUS:85149593257
SN - 2095-5545
VL - 12
JO - Light: Science and Applications
JF - Light: Science and Applications
IS - 1
M1 - 58
ER -