Abstract
Sorting it out: Applied in tandem, elastin-like polypeptides (ELPs, see scheme) and the Sortase A (SrtA) transpeptidase provide a method for chromatography-free purification of recombinant proteins and optional, site-specific conjugation of the protein to a small molecule (yellow). This system provides an efficient mechanism for generating bioactive proteins at high yields and purities.
Original language | English |
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Pages (from-to) | 3703-3708 |
Number of pages | 6 |
Journal | Angewandte Chemie - International Edition |
Volume | 52 |
Issue number | 13 |
DOIs | |
State | Published - 25 Mar 2013 |
Externally published | Yes |
Keywords
- Sortase A
- anticancer agents
- bioorganic chemistry
- biotechnology
- protein expression
ASJC Scopus subject areas
- Catalysis
- General Chemistry