Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides

Joseph J. Bellucci, Miriam Amiram, Jayanta Bhattacharyya, Dewey McCafferty, Ashutosh Chilkoti

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Sorting it out: Applied in tandem, elastin-like polypeptides (ELPs, see scheme) and the Sortase A (SrtA) transpeptidase provide a method for chromatography-free purification of recombinant proteins and optional, site-specific conjugation of the protein to a small molecule (yellow). This system provides an efficient mechanism for generating bioactive proteins at high yields and purities.

Original languageEnglish
Pages (from-to)3703-3708
Number of pages6
JournalAngewandte Chemie - International Edition
Volume52
Issue number13
DOIs
StatePublished - 25 Mar 2013
Externally publishedYes

Keywords

  • Sortase A
  • anticancer agents
  • bioorganic chemistry
  • biotechnology
  • protein expression

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