Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells

D. Baranes; F. T. Liu; E. Razin

doi:10.1016/0014-5793(86)81341-2

Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells

D. Baranes, F. T. Liu, E. Razin

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6 Scopus citations

Abstract

Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP₁ IP₂ and IP₃) could be detected 20 s after stimulation. The depletion of Ca²⁺ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP₃ under these conditions independent of the presence of extracellular Ca²⁺.

Original language	English
Pages (from-to)	64-68
Number of pages	5
Journal	FEBS Letters
Volume	206
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)81341-2
State	Published - 29 Sep 1986
Externally published	Yes

Keywords

(E-mast cell)
Inositol phosphate

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)81341-2

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title = "Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells",

abstract = "Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP1 IP2 and IP3) could be detected 20 s after stimulation. The depletion of Ca2+ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP3 under these conditions independent of the presence of extracellular Ca2+.",

keywords = "(E-mast cell), Inositol phosphate",

author = "D. Baranes and Liu, {F. T.} and E. Razin",

year = "1986",

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day = "29",

doi = "10.1016/0014-5793(86)81341-2",

language = "English",

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journal = "FEBS Letters",

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T1 - Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells

AU - Baranes, D.

AU - Liu, F. T.

AU - Razin, E.

PY - 1986/9/29

Y1 - 1986/9/29

N2 - Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP1 IP2 and IP3) could be detected 20 s after stimulation. The depletion of Ca2+ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP3 under these conditions independent of the presence of extracellular Ca2+.

AB - Stimulation of murine chondroitin sulfate E containing mast cells (E-MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP1 IP2 and IP3) could be detected 20 s after stimulation. The depletion of Ca2+ from the medium resulted in more than 80% reduction in β-hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP3 under these conditions independent of the presence of extracellular Ca2+.

KW - (E-mast cell)

KW - Inositol phosphate

UR - http://www.scopus.com/inward/record.url?scp=0022999155&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(86)81341-2

DO - 10.1016/0014-5793(86)81341-2

M3 - Article

AN - SCOPUS:0022999155

SN - 0014-5793

VL - 206

SP - 64

EP - 68

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Thrombin and IgE antigen induce formation of inositol phosphates by mouse E-mast cells

Abstract

Keywords

ASJC Scopus subject areas

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