Tomographic phase microscopy using optical tweezers

Mor Habaza, Barak Gilboa, Yael Roichman, Natan T. Shaked

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We review our technique for tomographic phase microscopy with optical tweezers [1]. This tomographic phase microscopy approach enables full 3-D refractive-index reconstruction. Tomographic phase microscopy measures quantitatively the 3-D distribution of refractive-index in biological cells. We integrated our external interferometric module with holographic optical tweezers for obtaining quantitative phase maps of biological samples from a wide range of angles. The close-tocommon-path, off-axis interferometric system enables a full-rotation tomographic acquisition of a single cell using holographic optical tweezers for trapping and manipulating with a desired array of traps, while acquiring phase information of a single cell from all different angles and maintaining the native surrounding medium. We experimentally demonstrated two reconstruction algorithms: the filtered back-projection method and the Fourier diffraction method for 3-D refractive index imaging of yeast cells.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2015
PublisherOptica Publishing Group (formerly OSA)
ISBN (Print)9781628417012
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2015 - Munich, Germany
Duration: 21 Jun 201525 Jun 2015

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2015
Country/TerritoryGermany
CityMunich
Period21/06/1525/06/15

Keywords

  • Holography
  • Image reconstruction techniques
  • Interference microscopy
  • Optical tweezers
  • Three dimensional image processing
  • Tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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