Topical treatment of cutaneous leishmaniasis in belize: In vitro and in vivo studies with Leishmania mexicana

J. El-On; F. Cawich; D. A. Evans; L. Weinrauch

doi:10.1016/0020-7519(93)90104-7

Topical treatment of cutaneous leishmaniasis in belize: In vitro and in vivo studies with Leishmania mexicana

J. El-On, F. Cawich, D. A. Evans, L. Weinrauch

Basic Sciences Divisions

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Strains of Leishmania mexicana isolated from Belizian patients were found to be highly susceptible to paromomycin sulphate (PR) treatment. This drug at 100 μg ml^-1 destroyed 85-99.5% of in vitro cultivated Leishmania promastigotes within 4 days of exposure to the drug. Leishmania promastigotes inoculated into the base of the tail of Balb/c mice caused the development of local lesions several weeks after infection. These lesions were totally cleared of parasites after 20 days of topical treatment with PR ointment, comprised of 15% paromomycin sulphate and 12% methylbenzethonium chloride in soft white paraffin. Similar results were also obtained with L. braziliensis infections. Isoenzyme analysis was found to be the method of choice for parasite strain identification. Excreted factor serotyping was only partially effective and promastigote agglutination gave negative results.

Original language	English
Pages (from-to)	121-127
Number of pages	7
Journal	International Journal for Parasitology
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/0020-7519(93)90104-7
State	Published - 1 Jan 1993

Keywords

Leishmania mexicana
identification
paromomycin
topical treatment

ASJC Scopus subject areas

Parasitology
Infectious Diseases

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10.1016/0020-7519(93)90104-7

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title = "Topical treatment of cutaneous leishmaniasis in belize: In vitro and in vivo studies with Leishmania mexicana",

abstract = "Strains of Leishmania mexicana isolated from Belizian patients were found to be highly susceptible to paromomycin sulphate (PR) treatment. This drug at 100 μg ml-1 destroyed 85-99.5% of in vitro cultivated Leishmania promastigotes within 4 days of exposure to the drug. Leishmania promastigotes inoculated into the base of the tail of Balb/c mice caused the development of local lesions several weeks after infection. These lesions were totally cleared of parasites after 20 days of topical treatment with PR ointment, comprised of 15% paromomycin sulphate and 12% methylbenzethonium chloride in soft white paraffin. Similar results were also obtained with L. braziliensis infections. Isoenzyme analysis was found to be the method of choice for parasite strain identification. Excreted factor serotyping was only partially effective and promastigote agglutination gave negative results.",

keywords = "Leishmania mexicana, identification, paromomycin, topical treatment",

author = "J. El-On and F. Cawich and Evans, {D. A.} and L. Weinrauch",

note = "Funding Information: Acknowledgements--Thsistu dyw as supportedb y the AID-CDR Grant No. DPE-5544-G-SS-7047-00. Paromomycin sulphate was kindly supplied by Parke Davis Warner Lambert,I taly and Farmitalia.C arlo Erba. Italy.",

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doi = "10.1016/0020-7519(93)90104-7",

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T2 - In vitro and in vivo studies with Leishmania mexicana

AU - El-On, J.

AU - Cawich, F.

AU - Evans, D. A.

AU - Weinrauch, L.

N1 - Funding Information: Acknowledgements--Thsistu dyw as supportedb y the AID-CDR Grant No. DPE-5544-G-SS-7047-00. Paromomycin sulphate was kindly supplied by Parke Davis Warner Lambert,I taly and Farmitalia.C arlo Erba. Italy.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Strains of Leishmania mexicana isolated from Belizian patients were found to be highly susceptible to paromomycin sulphate (PR) treatment. This drug at 100 μg ml-1 destroyed 85-99.5% of in vitro cultivated Leishmania promastigotes within 4 days of exposure to the drug. Leishmania promastigotes inoculated into the base of the tail of Balb/c mice caused the development of local lesions several weeks after infection. These lesions were totally cleared of parasites after 20 days of topical treatment with PR ointment, comprised of 15% paromomycin sulphate and 12% methylbenzethonium chloride in soft white paraffin. Similar results were also obtained with L. braziliensis infections. Isoenzyme analysis was found to be the method of choice for parasite strain identification. Excreted factor serotyping was only partially effective and promastigote agglutination gave negative results.

AB - Strains of Leishmania mexicana isolated from Belizian patients were found to be highly susceptible to paromomycin sulphate (PR) treatment. This drug at 100 μg ml-1 destroyed 85-99.5% of in vitro cultivated Leishmania promastigotes within 4 days of exposure to the drug. Leishmania promastigotes inoculated into the base of the tail of Balb/c mice caused the development of local lesions several weeks after infection. These lesions were totally cleared of parasites after 20 days of topical treatment with PR ointment, comprised of 15% paromomycin sulphate and 12% methylbenzethonium chloride in soft white paraffin. Similar results were also obtained with L. braziliensis infections. Isoenzyme analysis was found to be the method of choice for parasite strain identification. Excreted factor serotyping was only partially effective and promastigote agglutination gave negative results.

KW - Leishmania mexicana

KW - identification

KW - paromomycin

KW - topical treatment

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DO - 10.1016/0020-7519(93)90104-7

M3 - Article

AN - SCOPUS:0027511787

SN - 0020-7519

VL - 23

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EP - 127

JO - International Journal for Parasitology

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ER -

Topical treatment of cutaneous leishmaniasis in belize: In vitro and in vivo studies with Leishmania mexicana

Abstract

Keywords

ASJC Scopus subject areas

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