Topical treatment of cutaneous leishmaniasis in belize: In vitro and in vivo studies with Leishmania mexicana

J. El-On, F. Cawich, D. A. Evans, L. Weinrauch

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15 Scopus citations

Abstract

Strains of Leishmania mexicana isolated from Belizian patients were found to be highly susceptible to paromomycin sulphate (PR) treatment. This drug at 100 μg ml-1 destroyed 85-99.5% of in vitro cultivated Leishmania promastigotes within 4 days of exposure to the drug. Leishmania promastigotes inoculated into the base of the tail of Balb/c mice caused the development of local lesions several weeks after infection. These lesions were totally cleared of parasites after 20 days of topical treatment with PR ointment, comprised of 15% paromomycin sulphate and 12% methylbenzethonium chloride in soft white paraffin. Similar results were also obtained with L. braziliensis infections. Isoenzyme analysis was found to be the method of choice for parasite strain identification. Excreted factor serotyping was only partially effective and promastigote agglutination gave negative results.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalInternational Journal for Parasitology
Volume23
Issue number1
DOIs
StatePublished - 1 Jan 1993

Keywords

  • Leishmania mexicana
  • identification
  • paromomycin
  • topical treatment

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