Trace metals supplementation enhanced microbiota and biohythane production by two-stage thermophilic fermentation

Chonticha Mamimin, Maraike Probst, María Gómez-Brandón, Sabine Marie Podmirseg, Heribert Insam, Alissara Reungsang, Sompong O-Thong

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The effect of trace metals supplementation into palm oil mill effluent on biohythane production and responsible microbial communities in thermophilic two-stage anaerobic fermentation was investigated. High biohythane yields were linked to Ni/Co/Fe supplementation (10, 6 and 20 mg L−1, respectively) with maximum H2 and CH4 yields of 139 mL H2 gVS−1 and 454 mL CH4 gVS−1, respectively. The Ni/Co/Fe supplementation resulted in higher numbers of Bacillus sp., Clostridium sp. and Thermoanaerobacterium sp. together with increasing hydrogenase expression level leading to increasing hydrogen yields of 90.4%. The numbers of Methanosarcina, Methanomassiliicoccus, and Methanoculleus were enhanced by Ni/Co/Fe addition, accompanied by 21.7% higher methane yields. No correlation between methyl coenzyme-M reductase expression level and methane yields was observed. The Ni/Co/Fe supplementation improved gas production in the two-stage biohythane process via enhancing a number of viable hydrogen-producing bacteria together with hydrogenase activity in H2 stage and enhancing number methanogens in the CH4 stage.

Original languageEnglish
Pages (from-to)3325-3338
Number of pages14
JournalInternational Journal of Hydrogen Energy
Issue number6
StatePublished - 4 Feb 2019
Externally publishedYes


  • Biohythane production
  • Hydrogenase (hydA) gene
  • Next generation sequencing
  • Trace elements
  • Two-stage fermentation

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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