Transcription and translation of the chloroplast atpB-gene and assembly of ATP synthase subunit β

Stefan Leu; Dvorah Weinberg; Allan Michaels

doi:10.1016/0014-5793(90)81114-4

Transcription and translation of the chloroplast atpB-gene and assembly of ATP synthase subunit β

Stefan Leu
, Dvorah Weinberg
, Allan Michaels

The French Associates Institute for Agriculture and Biotechnology of Drylands

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In vitro transcription and subsequent translation of the cloned Chlamydomonas chloroplast atpB gene was used to study assembly ofATP synthase. Translation in the presence of thylakoids resulted in association of the β subunit with the membrane. The in vitro synthesized polypeptide bound to the membrane copurified with CF₁ on sucrose gradients. This provides more evidence for the self-assembly of CF₁.

Original language	English
Pages (from-to)	41-44
Number of pages	4
Journal	FEBS Letters
Volume	269
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)81114-4
State	Published - 20 Aug 1990

Keywords

ATP synthase
CF assembly
Chloroplast
Translation
atpB-gene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(90)81114-4

Cite this

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abstract = "In vitro transcription and subsequent translation of the cloned Chlamydomonas chloroplast atpB gene was used to study assembly ofATP synthase. Translation in the presence of thylakoids resulted in association of the β subunit with the membrane. The in vitro synthesized polypeptide bound to the membrane copurified with CF1 on sucrose gradients. This provides more evidence for the self-assembly of CF1.",

keywords = "ATP synthase, CF assembly, Chloroplast, Translation, atpB-gene",

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AU - Michaels, Allan

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Y1 - 1990/8/20

N2 - In vitro transcription and subsequent translation of the cloned Chlamydomonas chloroplast atpB gene was used to study assembly ofATP synthase. Translation in the presence of thylakoids resulted in association of the β subunit with the membrane. The in vitro synthesized polypeptide bound to the membrane copurified with CF1 on sucrose gradients. This provides more evidence for the self-assembly of CF1.

AB - In vitro transcription and subsequent translation of the cloned Chlamydomonas chloroplast atpB gene was used to study assembly ofATP synthase. Translation in the presence of thylakoids resulted in association of the β subunit with the membrane. The in vitro synthesized polypeptide bound to the membrane copurified with CF1 on sucrose gradients. This provides more evidence for the self-assembly of CF1.

KW - ATP synthase

KW - CF assembly

KW - Chloroplast

KW - Translation

KW - atpB-gene

UR - https://www.scopus.com/pages/publications/0025111054

U2 - 10.1016/0014-5793(90)81114-4

DO - 10.1016/0014-5793(90)81114-4

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AN - SCOPUS:0025111054

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IS - 1

ER -

Transcription and translation of the chloroplast atpB-gene and assembly of ATP synthase subunit β

Abstract

Keywords

ASJC Scopus subject areas

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