Transcriptome analysis identifies regulators of hematopoietic stem and progenitor cells

The Immunological Genome Project Consortium

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Summary Hematopoietic stem cells (HSCs) maintain blood homeostasis and are the functional units of bone marrow transplantation. To improve the molecular understanding of HSCs and their proximal progenitors, we performed transcriptome analysis within the context of the ImmGen Consortium data set. Gene sets that define steady-state and mobilized HSCs, as well as hematopoietic stem and progenitor cells (HSPCs), were determined. Genes involved in transcriptional regulation, including a group of putative transcriptional repressors, were identified in multipotent progenitors and HSCs. Proximal promoter analyses combined with ImmGen module analysis identified candidate regulators of HSCs. Enforced expression of one predicted regulator, Hlf, in diverse HSPC subsets led to extensive self-renewal activity ex vivo. These analyses reveal unique insights into the mechanisms that control the core properties of HSPCs.

Original languageEnglish
Pages (from-to)266-280
Number of pages15
JournalStem Cell Reports
Volume1
Issue number3
DOIs
StatePublished - 10 Sep 2013

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