TY - JOUR
T1 - Transfer of the movement protein gene between two tobamoviruses
T2 - Influence on local lesion development
AU - Nejidat, Ali
AU - Cellier, Françoise
AU - Holt, Curtis A.
AU - Gafny, Ron
AU - Eggenberger, Alan L.
AU - Beachy, Roger N.
N1 - Funding Information:
Fhis work was supported by grants from the National Institutes of Health (A127161), the National Science Foundation (DMB 8717012), and the Monsanto Company. A.N. was supported in part by a Fullbright postdoctoral fellowship ; F .C . was supported in part by a predoctoral fellowship from INRA, Versailles, France . The oligonucleotides used for in vitro mutagenesis were kindly provided by Ms . K . Kavka, Monsanto Co .
PY - 1991/1/1
Y1 - 1991/1/1
N2 - The effects of transfer of the movement gene between the tobamoviruses tobacco mosaic virus (TMV) and tobacco mild green mosaic virus (TMGMV) were studied. The movement protein (MP) gene of TMGMV was cloned into an infectious cDNA of TMV to build the recombinant virus V23. V23, like TMV and TMGMV, caused systemic infection in Nicotiana tabacum Xanthi. In N. sylvestris V23 and TMV spread systemically although TMGMV produces necrotic local lesions on this host. V23 and TMV cause systemic infection on tomato plants while TMGMV does not infect tomato. In Xanthi nc plants, V23 produced necrotic local lesions similar in size to those produced by TMGMV. On the other hand in transgenic Xanthi nc tobacco plants that express a gene encoding the MP of TMV the necrotic lesions produced by V23 and TMGMV were similar in size to those produced by TMV. These results indicate that the size of necrotic lesions produced by TMGMV and TMV on Xanthi nc plants is influenced by the MP gene.
AB - The effects of transfer of the movement gene between the tobamoviruses tobacco mosaic virus (TMV) and tobacco mild green mosaic virus (TMGMV) were studied. The movement protein (MP) gene of TMGMV was cloned into an infectious cDNA of TMV to build the recombinant virus V23. V23, like TMV and TMGMV, caused systemic infection in Nicotiana tabacum Xanthi. In N. sylvestris V23 and TMV spread systemically although TMGMV produces necrotic local lesions on this host. V23 and TMV cause systemic infection on tomato plants while TMGMV does not infect tomato. In Xanthi nc plants, V23 produced necrotic local lesions similar in size to those produced by TMGMV. On the other hand in transgenic Xanthi nc tobacco plants that express a gene encoding the MP of TMV the necrotic lesions produced by V23 and TMGMV were similar in size to those produced by TMV. These results indicate that the size of necrotic lesions produced by TMGMV and TMV on Xanthi nc plants is influenced by the MP gene.
UR - http://www.scopus.com/inward/record.url?scp=0026054569&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(91)90036-B
DO - 10.1016/0042-6822(91)90036-B
M3 - Article
AN - SCOPUS:0026054569
SN - 0042-6822
VL - 180
SP - 318
EP - 326
JO - Virology
JF - Virology
IS - 1
ER -