Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells

Gabrielle Nina Thibodeaux, Xiang Liang, Kathryn Moncivais, Aiko Umeda, Oded Singer, Lital Alfonta, Zhiwen Jonathan Zhang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A previously engineered Methanocaldococcus jannaschii tRNACUrA-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting tRNACUrA-tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent protein, GFP, and in a foldon protein in mammalian cells. The methodology reported here will allow rapid transformation of the much larger collection of existing tyrosyl-tRNA synthetases that were already evolved for the incorporation of an array of over 50 unnatural amino acids into proteins in Escherichia coli into proteins in mammalian cells. Thus we will be able to introduce a large array of possibilities for protein modifications in mammalian cells.

Original languageEnglish
Article numbere11263
JournalPLoS ONE
Volume5
Issue number6
DOIs
StatePublished - 11 Aug 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Agricultural and Biological Sciences (all)
  • General

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