Translation Inhibition by Phosphorothioate Oligodeoxynucleotides in Cell-Free Systems

Mridul K. Ghosh; Krishnakali Ghosh; Jack S. Cohen

doi:10.1089/ard.1992.2.111

Translation Inhibition by Phosphorothioate Oligodeoxynucleotides in Cell-Free Systems

Mridul K. Ghosh, Krishnakali Ghosh, Jack S. Cohen

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35 Scopus citations

Abstract

A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti—β-globin sequence in cell-free systems using β-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (>4 μM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for RNase H—mediated translation inhibition.

Original language	English
Pages (from-to)	111-118
Number of pages	8
Journal	Antisense Research and Development
Volume	2
Issue number	2
DOIs	https://doi.org/10.1089/ard.1992.2.111
State	Published - 1 Jan 1992
Externally published	Yes

ASJC Scopus subject areas

Genetics

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abstract = "A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti—β-globin sequence in cell-free systems using β-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (>4 μM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for RNase H—mediated translation inhibition.",

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AU - Cohen, Jack S.

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AB - A detailed comparison was made of the concentration dependence of translation inhibition by phosphorothioate and phosphodiester oligodeoxynucleotides of the same anti—β-globin sequence in cell-free systems using β-globin mRNA and unrelated mRNAs as controls. The results confirm that at low concentrations the phosphorothioate oligomer is more potent as an antisense compound, while at higher concentration (>4 μM) it exhibits more nonspecific inhibition than the phosphodiester oligomer for RNase H—mediated translation inhibition.

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Translation Inhibition by Phosphorothioate Oligodeoxynucleotides in Cell-Free Systems

Abstract

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