TY - JOUR
T1 - Transplantation of frozen-thawed late-gestational-age human fetal ovaries into immunodeficient mice
AU - Abir, Ronit
AU - Biron-Shental, Tal
AU - Orvieto, Raoul
AU - Garor, Roni
AU - Krissi, Haim
AU - Fisch, Benjamin
N1 - Funding Information:
Partially supported by a research grant from the Israel Ministry of Health and Justice Department (to B.F. and R.A.).
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Objective: To compare the development of human fetal follicles from late-gestational-age fetuses frozen-thawed gradually and slowly with dimethylsulfoxide (DMSO) or 1,2 propanediol (PROH) and sucrose after renal grafting into follicle stimulating hormone-treated immunodeficient mice. Design: Controlled histologic study of grafted human fetal ovaries. Setting: Major tertiary care academic center. Patient(s): Eleven women undergoing pregnancy termination at 22 to 33 gestational weeks. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis and immunohistochemistry for proliferating cell nuclear antigen (PCNA). Result(s): Only follicles from samples frozen-thawed with PROH developed to secondary and antral stages 4 to 6 months after grafting, with PCNA expression in their granulosa cells. However, the number of surviving/developing follicles per section was very low (4-25 per graft), compared with 71 to 406 follicles in pretransplantation samples. Graft recovery was very high, with similar rates for transplants frozen-thawed with PROH and DMSO. Normal ovarian structure after grafting was identified only in the PROH frozen-thawed grafts. In deteriorated grafts, frozen-thawed with either DMSO or PROH, net-like hollows replaced follicles, whereas tubule-like structures were only identified in DMSO frozen-thawed grafts. Conclusion(s): This is the first report of the development of late-pregnancy-stage human fetal follicles in immunodeficient mice. PROH freezing-thawing supported development and survival better than DMSO. However, the low follicular survival points to the urgent need for efficient methods to enhance vascularization rate and prevent ischemia.
AB - Objective: To compare the development of human fetal follicles from late-gestational-age fetuses frozen-thawed gradually and slowly with dimethylsulfoxide (DMSO) or 1,2 propanediol (PROH) and sucrose after renal grafting into follicle stimulating hormone-treated immunodeficient mice. Design: Controlled histologic study of grafted human fetal ovaries. Setting: Major tertiary care academic center. Patient(s): Eleven women undergoing pregnancy termination at 22 to 33 gestational weeks. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis and immunohistochemistry for proliferating cell nuclear antigen (PCNA). Result(s): Only follicles from samples frozen-thawed with PROH developed to secondary and antral stages 4 to 6 months after grafting, with PCNA expression in their granulosa cells. However, the number of surviving/developing follicles per section was very low (4-25 per graft), compared with 71 to 406 follicles in pretransplantation samples. Graft recovery was very high, with similar rates for transplants frozen-thawed with PROH and DMSO. Normal ovarian structure after grafting was identified only in the PROH frozen-thawed grafts. In deteriorated grafts, frozen-thawed with either DMSO or PROH, net-like hollows replaced follicles, whereas tubule-like structures were only identified in DMSO frozen-thawed grafts. Conclusion(s): This is the first report of the development of late-pregnancy-stage human fetal follicles in immunodeficient mice. PROH freezing-thawing supported development and survival better than DMSO. However, the low follicular survival points to the urgent need for efficient methods to enhance vascularization rate and prevent ischemia.
KW - DMSO
KW - Human fetal ovaries
KW - PCNA
KW - PROH
KW - developing follicles
KW - primordial follicles
UR - http://www.scopus.com/inward/record.url?scp=67749091277&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2008.06.032
DO - 10.1016/j.fertnstert.2008.06.032
M3 - Article
AN - SCOPUS:67749091277
SN - 0015-0282
VL - 92
SP - 770
EP - 777
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 2
ER -