TY - JOUR
T1 - Triacylglycerols of the red microalga Porphyridium cruentum can contribute to the biosynthesis of eukaryotic galactolipids
AU - Khozin-Goldberg, Inna
AU - Yu, Hu Zheng
AU - Adlerstein, Daniel
AU - Didi-Cohen, Shoshana
AU - Heimer, Yair M.
AU - Cohen, Zvi
N1 - Funding Information:
This research was supported in part by the Israeli ministry of art and sciences. I.K-G. was supported in part by a fellowship from the Israeli ministry of absorption. Contribution No. 128 from the Laboratory for Microalgal Biotechnology, Jacob Blaustein Institute for Desert Research.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - A mutant of the red microalga Porphyridium cruentum was selected on the basis of impaired growth at suboptimal temperatures (15 vs. 25°C). Fatty acid and lipid analyses revealed diminished proportions of eicosapentaenoic acid (from 41 to 30%) and of the eukaryotic molecular species (from 38 to 28% of monogalactosyldiacylglycerol (MGDG) and elevated proportion (10 vs. 2%) of triacylglycerols (TAG) in the mutant, as compared with the wild type. Pulse labeling of the wild type cells with radioactive fatty acid precursors indicated an initial incorporation of the fatty acids into phosphatidylcholine (PC) and TAG. Following the pulse, the label of PC and TAG decreased with time (from 25 to 5% of the total dpm in TAG) while that of chloroplastic polar lipids, mainly MGDG, continued to increase. In the mutant, however, the labeling of TAG after the pulse was higher (30% of the total dpm) than that of the wild type and decreased only slightly to 20%. This may indicate that in P. cruentum, TAG can contribute to the biosynthesis of eukaryotic species of MGDG.
AB - A mutant of the red microalga Porphyridium cruentum was selected on the basis of impaired growth at suboptimal temperatures (15 vs. 25°C). Fatty acid and lipid analyses revealed diminished proportions of eicosapentaenoic acid (from 41 to 30%) and of the eukaryotic molecular species (from 38 to 28% of monogalactosyldiacylglycerol (MGDG) and elevated proportion (10 vs. 2%) of triacylglycerols (TAG) in the mutant, as compared with the wild type. Pulse labeling of the wild type cells with radioactive fatty acid precursors indicated an initial incorporation of the fatty acids into phosphatidylcholine (PC) and TAG. Following the pulse, the label of PC and TAG decreased with time (from 25 to 5% of the total dpm in TAG) while that of chloroplastic polar lipids, mainly MGDG, continued to increase. In the mutant, however, the labeling of TAG after the pulse was higher (30% of the total dpm) than that of the wild type and decreased only slightly to 20%. This may indicate that in P. cruentum, TAG can contribute to the biosynthesis of eukaryotic species of MGDG.
UR - http://www.scopus.com/inward/record.url?scp=0033859543&partnerID=8YFLogxK
U2 - 10.1007/S11745-000-0597-8
DO - 10.1007/S11745-000-0597-8
M3 - Article
AN - SCOPUS:0033859543
SN - 0024-4201
VL - 35
SP - 881
EP - 889
JO - Lipids
JF - Lipids
IS - 8
ER -