Tumor vaccination by Salmonella typhimurium after transformation with a eukaryotic expression vector in mice: Impact of a Salmonella typhimurium gene interfering with MHC class I presentation

Susanne Hummel, Ron N. Apte, Udi Qimron, Mario Vitacolonna, Angel Porgador, Margot Zöller

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Transformed attenuated Salmonella typhimurium (ST) have been suggested as an efficient means of tumor vaccination. However, ST themselves might be immunosuppressive, and the question has arisen as to whether this impedes vaccination efficacy even if ST are transformed with a eukaryotic expression vector such that "tumor antigen" will be transcribed by the host. The question was evaluated using a mutant SL7207, where the yej operon, which interferes with MHC I-mediated presentation, had been inactivated (SL7207ΔyejE). Mice were vaccinated with SL7207 or SL7207ΔyejE transformed with a eukaryotic expression vector carrying the lacZ or the gp100 gene and later received lacZ-transfected RENCA or YC8 or gp100-expressing B16F1 tumor cells. In vaccinated mice, tumor growth started with a delay and some animals remained tumor-free; however, the tumor growth rate remained unaltered. No significant difference was seen between SL7207ΔyejE versus SL7207 vaccinated mice. The latter finding contrasted with ex vivo analyses where vaccination with SL7207ΔyejE, compared with SL7207, induced a significantly stronger response, including nonadaptive defense mechanisms. The failure to detect a superior vaccination efficacy of SL7207ΔyejE in vivo could be attributed to a stronger effect of the yej operon on MHC-mediated antigen presentation when driven by a prokaryotic promoter. Also, additional Salmonella genes apparently interfere with maintenance of a sustained immune response. Thus, the immunosuppressive yej operon affects innate and adaptive immunity. However, when ST are carriers for eukaryotic-expressed tumor antigens, yej does not severely hamper induction of an immune response.

Original languageEnglish
Pages (from-to)467-479
JournalJournal of Immunotherapy
Volume28
Issue number5
DOIs
StatePublished - 1 Jan 2005

Keywords

  • Immunosuppression
  • Salmonella typhimurium
  • Tumor vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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