Two ITS forms co-inhabiting a single genet of an isolate of Terfezia boudieri (Ascomycotina), a desert truffle

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Two fruit-bodies of Terfezia boudieri Chatin, each exhibiting a mixture of two ITS-RFLP profiles, were found in the Negev desert of Israel. A mycelial culture obtained from glebal out-growth maintained the double profile, as did proliferating cultures established using single hyphae isolated from the original cultures. The main difference between the two ITS variants lies in a 21 bp deletion in the smaller variant. The question whether both variants are contained within a single nucleus or occupy different nuclei sharing the same cytoplasm is discussed.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalAntonie van Leeuwenhoek
Issue number2
StatePublished - 1 Feb 2004


  • Heteroduplex
  • Heterokaryon
  • Intraspecific variability
  • Terfezia


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