Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy

Zhengyang Zhang; Samuel J. Kenny; Margaret Hauser; Wan Li; Ke Xu

doi:10.1038/nmeth.3528

Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy

Zhengyang Zhang, Samuel J. Kenny, Margaret Hauser, Wan Li, Ke Xu

Research output: Contribution to journal › Article › peer-review

211 Scopus citations

Abstract

By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of ∼10⁶ single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk-free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.

Original language	English
Pages (from-to)	935-938
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.3528
State	Published - 29 Sep 2015
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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abstract = "By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of ∼106 single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk-free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.",

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Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy

Abstract

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