Ultrasensitive and specific electronic transduction of DNA sensing processes

I Willner, F Patolsky, L Alfonta, A Lichtenstein, B Willner

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Amplified electronic transduction of DNA sensing is accomplished by the application of a biocatalytic probe that precipitates an insoluble product on the transducer as a result of the DNA sensing. Alternative amplification of DNA sensing is achieved by the use of functionalized liposomes that bind to the electrode upon the sensing process. Single base mutations in DNA are detected by the polymerase-induced coupling of a biotinylated base complementary to the mismatch site, followed by the biocatalyzed precipitation of an insoluble product on the transducer. DNA biosensors of unprecedented sensitivity and specificity are designed. Faradaic impedance spectroscopy and microgravimetric quartz-crystal-microbalance measurements are used for the electronic transduction of the DNA sensing.
Original languageEnglish
Title of host publicationNovel Approaches in Biosensors and Rapid Diagnostic Assays
EditorsZvi Liron, Avraham Bromberg, Morly Fisher
Place of PublicationBoston, MA
PublisherSpringer
Pages47-78
Number of pages32
ISBN (Electronic)9781461512318
ISBN (Print)9781461354529
DOIs
StatePublished - 2001

Keywords

  • DNA
  • bioelectronics
  • biosensors
  • gene detection
  • genetic disorders
  • impedance spectroscopy
  • Quartz-Crystal-Microbalance
  • liposomes.

Fingerprint

Dive into the research topics of 'Ultrasensitive and specific electronic transduction of DNA sensing processes'. Together they form a unique fingerprint.

Cite this