TY - JOUR
T1 - Ultrastructure of synapses in the lateral line canal organ
AU - Benshalom, G.
AU - Flock, Å
N1 - Funding Information:
We wish to thank Bengt Hedberg for skilful photographic work, and Maud Hoffstedt for help with freeze-fracture specimen. One of us (G. B.) was supported by a guest-scholarship from The Swedish Institute. This work has been supported by grants from the Swedish Medical Research Council (04X-02461). and the King Gustaf V Memorial Fund.
PY - 1980/1/1
Y1 - 1980/1/1
N2 - The ultrastructure of afferent and efferent synapses on hair cells in the lateral line canal organ of the fish Lota Iota was studied, utilizing various fixation and staining techniques. New information was obtained about membrane-associated material, such as the presynaptic body and postsynaptic densities, by examining glutaral-dehyde-fixed material not subjected to osmication. Contrast was instead obtained either by section staining with uranyl acetate and lead citrate or by block impregnation with phosphotungstic acid (PTA). PTA-staining enhances the postsynaptic membrane of both the afferent and efferent synapses. It also stains the presynaptic dense projections at the efferent synapse and in a differential fashion, the afferent synaptic body. Section staining reveals a substructure in the feet of the afferent synaptic body, faintly seen but masked in osmicated tissue.
AB - The ultrastructure of afferent and efferent synapses on hair cells in the lateral line canal organ of the fish Lota Iota was studied, utilizing various fixation and staining techniques. New information was obtained about membrane-associated material, such as the presynaptic body and postsynaptic densities, by examining glutaral-dehyde-fixed material not subjected to osmication. Contrast was instead obtained either by section staining with uranyl acetate and lead citrate or by block impregnation with phosphotungstic acid (PTA). PTA-staining enhances the postsynaptic membrane of both the afferent and efferent synapses. It also stains the presynaptic dense projections at the efferent synapse and in a differential fashion, the afferent synaptic body. Section staining reveals a substructure in the feet of the afferent synaptic body, faintly seen but masked in osmicated tissue.
UR - http://www.scopus.com/inward/record.url?scp=0018941993&partnerID=8YFLogxK
U2 - 10.3109/00016488009131712
DO - 10.3109/00016488009131712
M3 - Article
AN - SCOPUS:0018941993
SN - 0001-6489
VL - 90
SP - 161
EP - 174
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - 1-6
ER -