Ultraviolet radiation induces stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal stress signal: A 15N NMR study

E. B.I. Monselise; A. Levkovitz; D. Kost

doi:10.1111/plb.12198

Ultraviolet radiation induces stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal stress signal: A ¹⁵N NMR study

E. B.I. Monselise
, A. Levkovitz
, D. Kost

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Analysis with ¹⁵N NMR revealed that alanine, a universal cellular stress signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in ¹⁵N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.

Original language	English
Pages (from-to)	101-107
Number of pages	7
Journal	Plant Biology
Volume	17
Issue number	s1
DOIs	https://doi.org/10.1111/plb.12198
State	Published - 1 Jan 2015

Keywords

D-alanine accumulation
Etiolated plants
N-NMR analysis
Nitrogen assimilation
Radical scavenger
Vitamin C

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Plant Science

Access to Document

10.1111/plb.12198

Cite this

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title = "Ultraviolet radiation induces stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal stress signal: A 15N NMR study",

abstract = "Analysis with 15N NMR revealed that alanine, a universal cellular stress signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in 15N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.",

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author = "Monselise, \{E. B.I.\} and A. Levkovitz and D. Kost",

note = "Publisher Copyright: {\textcopyright} 2014 German Botanical Society and The Royal Botanical Society of the Netherlands.",

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AU - Kost, D.

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AB - Analysis with 15N NMR revealed that alanine, a universal cellular stress signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in 15N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.

KW - D-alanine accumulation

KW - Etiolated plants

KW - N-NMR analysis

KW - Nitrogen assimilation

KW - Radical scavenger

KW - Vitamin C

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