TY - JOUR
T1 - Uniform and Easy-To-Prepare Glycopolymer-Brush Interface for Rapid Protein (Anti-)Adhesion Sensing
AU - Li, Junji
AU - Tian, Xiao Yang
AU - Zong, Li Ping
AU - Zhang, Qiang
AU - Zhang, Xue Ji
AU - Marks, Robert
AU - Cosnier, Serge
AU - Shan, Dan
N1 - Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/9/4
Y1 - 2019/9/4
N2 - Glycopolymers have emerged as powerful and versatile glycan analogues for the investigation of cellular signal transduction. In this study, a layer of the glycopolymer-brush (GlyB) interface was functionalized on the surface of gold substrates. In order to enhance the capability and accessibility of this transducer interface, a combined protocol of copper(0)-mediated living radical polymerization (Cu(0)-LRP) with subsequent "CuAAC" click reaction was utilized to synthesize a set of novel glycopolymer precursors with a tunable scaffold structure and pyranose ligands. The resulting glycopolymer exhibited a fine-tuned molecular weight with a minor dispersity of 1.27. Through surface plasmon resonance (SPR) analysis, various GlyB interfaces exhibiting different saccharide moieties (glucose, mannose, and galactose) were examined to study their adhesion or antiadhesion potential toward three types of proteins, concanavalin A, bovine serum albumin, and peanut agglutinin (PNA). The strong affinity between poly(galactose) and PNA was further employed to construct a proof-of-concept aggregation-mediated sensing system. This minimal naked-eye sensor that consisted of only two substances, namely, gold nanoparticles and glycopolymers, was characterized and tested for its potential in protein quantification.
AB - Glycopolymers have emerged as powerful and versatile glycan analogues for the investigation of cellular signal transduction. In this study, a layer of the glycopolymer-brush (GlyB) interface was functionalized on the surface of gold substrates. In order to enhance the capability and accessibility of this transducer interface, a combined protocol of copper(0)-mediated living radical polymerization (Cu(0)-LRP) with subsequent "CuAAC" click reaction was utilized to synthesize a set of novel glycopolymer precursors with a tunable scaffold structure and pyranose ligands. The resulting glycopolymer exhibited a fine-tuned molecular weight with a minor dispersity of 1.27. Through surface plasmon resonance (SPR) analysis, various GlyB interfaces exhibiting different saccharide moieties (glucose, mannose, and galactose) were examined to study their adhesion or antiadhesion potential toward three types of proteins, concanavalin A, bovine serum albumin, and peanut agglutinin (PNA). The strong affinity between poly(galactose) and PNA was further employed to construct a proof-of-concept aggregation-mediated sensing system. This minimal naked-eye sensor that consisted of only two substances, namely, gold nanoparticles and glycopolymers, was characterized and tested for its potential in protein quantification.
KW - Cu(0)-LRP
KW - glycopolymer
KW - protein-induced aggregation
KW - surface plasmon resonance (SPR)
KW - transducer interface
UR - http://www.scopus.com/inward/record.url?scp=85071784276&partnerID=8YFLogxK
U2 - 10.1021/acsami.9b08566
DO - 10.1021/acsami.9b08566
M3 - Article
C2 - 31397991
AN - SCOPUS:85071784276
SN - 1944-8244
VL - 11
SP - 32366
EP - 32372
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 35
ER -