TY - JOUR
T1 - Unraveling the promise and limitations of CRISPR/Cas system in natural product research
T2 - Approaches and challenges
AU - Biswas, Protha
AU - Anand, Uttpal
AU - Ghorai, Mimosa
AU - Pandey, Devendra Kumar
AU - Jha, Niraj Kumar
AU - Behl, Tapan
AU - Kumar, Manoj
AU - Chauhan, Radha
AU - Shekhawat, Mahipal S.
AU - Dey, Abhijit
N1 - Publisher Copyright:
© 2021 Wiley-VCH GmbH.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - An incredible array of natural products is produced by plants that serve several ecological functions, including protecting them from herbivores and microbes, attracting pollinators, and dispersing seeds. In addition to their obvious medical applications, natural products serve as flavoring agents, fragrances, and many other uses by humans. With the increasing demand for natural products and the development of various gene engineering systems, researchers are trying to modify the plant genome to increase the biosynthetic pathway of the compound of interest or blocking the pathway of unwanted compound synthesis. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has had widespread success in genome editing due to the system's high efficiency, ease of use, and accuracy which revolutionized the genome editing system in living organisms. This study highlights the method of the CRISPR/Cas system, its application in different organisms including microbes, algae, fungi, and also higher plants in natural product research, and its shortcomings and future prospects.
AB - An incredible array of natural products is produced by plants that serve several ecological functions, including protecting them from herbivores and microbes, attracting pollinators, and dispersing seeds. In addition to their obvious medical applications, natural products serve as flavoring agents, fragrances, and many other uses by humans. With the increasing demand for natural products and the development of various gene engineering systems, researchers are trying to modify the plant genome to increase the biosynthetic pathway of the compound of interest or blocking the pathway of unwanted compound synthesis. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has had widespread success in genome editing due to the system's high efficiency, ease of use, and accuracy which revolutionized the genome editing system in living organisms. This study highlights the method of the CRISPR/Cas system, its application in different organisms including microbes, algae, fungi, and also higher plants in natural product research, and its shortcomings and future prospects.
KW - CRISPR/Cas
KW - gene modification
KW - genetic engineering
KW - genome editing
KW - metabolic engineering
KW - natural products
UR - http://www.scopus.com/inward/record.url?scp=85124490412&partnerID=8YFLogxK
U2 - 10.1002/biot.202100507
DO - 10.1002/biot.202100507
M3 - Review article
C2 - 34882991
AN - SCOPUS:85124490412
SN - 1860-6768
VL - 17
JO - Biotechnology Journal
JF - Biotechnology Journal
IS - 7
M1 - 2100507
ER -