Unusual alternative splicing within the human kallikrein genes KLK2 and KLK3 gives rise to novel prostate-specific proteins

Anat David, Nicola Mabjeesh, Idit Azar, Sharon Biton, Sharon Engel, Jeanne Bernstein, Jacob Romano, Yoav Avidor, Tova Waks, Zelig Eshhar, Salomon Z. Langer, Beatriz Lifschitz-Mercer, Haim Matzkin, Galit Rotman, Amir Toporik, Kinneret Savitsky, Liat Mintz

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Prostate-specific antigen (PSA) and human kallikrein 2 are closely related products of the human kallikrein genes KLK3 and KLK2, respectively. Both PSA and human kallikrein 2 are produced and secreted in the prostate and have important applications in the diagnosis of prostate cancer. We report here the identification of unusual mRNA splice variants of the KLK2 and KLK3 genes that result from inclusion of intronic sequences adjacent to the first exon. The novel proteins encoded by these transcripts, named PSA-linked molecule (PSA-LM) and hK2-linked molecule (K-LM), share only the signal peptide with the original protein product of the respective gene. The mature proteins are entirely different and bear no similarity to the kallikrein family or to other proteins in the databases. As is the case with PSA, PSA-LM is expressed in the secretory epithelial cells of the prostate and is up-regulated in response to androgenic stimulation. A similar pattern of expression is suggested for K-LM.

Original languageEnglish
Pages (from-to)18084-18090
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number20
DOIs
StatePublished - 17 May 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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