TY - JOUR
T1 - Unusual Two-Step Assembly of a Minimalistic Dipeptide-Based Functional Hypergelator
AU - Chakraborty, Priyadarshi
AU - Tang, Yiming
AU - Yamamoto, Tomoya
AU - Yao, Yifei
AU - Guterman, Tom
AU - Zilberzwige-Tal, Shai
AU - Adadi, Nofar
AU - Ji, Wei
AU - Dvir, Tal
AU - Ramamoorthy, Ayyalusamy
AU - Wei, Guanghong
AU - Gazit, Ehud
N1 - Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Self-assembled peptide hydrogels represent the realization of peptide nanotechnology into biomedical products. There is a continuous quest to identify the simplest building blocks and optimize their critical gelation concentration (CGC). Herein, a minimalistic, de novo dipeptide, Fmoc-Lys(Fmoc)-Asp, as an hydrogelator with the lowest CGC ever reported, almost fourfold lower as compared to that of a large hexadecapeptide previously described, is reported. The dipeptide self-assembles through an unusual and unprecedented two-step process as elucidated by solid-state NMR and molecular dynamics simulation. The hydrogel is cytocompatible and supports 2D/3D cell growth. Conductive composite gels composed of Fmoc-Lys(Fmoc)-Asp and a conductive polymer exhibit excellent DNA binding. Fmoc-Lys(Fmoc)-Asp exhibits the lowest CGC and highest mechanical properties when compared to a library of dipeptide analogues, thus validating the uniqueness of the molecular design which confers useful properties for various potential applications.
AB - Self-assembled peptide hydrogels represent the realization of peptide nanotechnology into biomedical products. There is a continuous quest to identify the simplest building blocks and optimize their critical gelation concentration (CGC). Herein, a minimalistic, de novo dipeptide, Fmoc-Lys(Fmoc)-Asp, as an hydrogelator with the lowest CGC ever reported, almost fourfold lower as compared to that of a large hexadecapeptide previously described, is reported. The dipeptide self-assembles through an unusual and unprecedented two-step process as elucidated by solid-state NMR and molecular dynamics simulation. The hydrogel is cytocompatible and supports 2D/3D cell growth. Conductive composite gels composed of Fmoc-Lys(Fmoc)-Asp and a conductive polymer exhibit excellent DNA binding. Fmoc-Lys(Fmoc)-Asp exhibits the lowest CGC and highest mechanical properties when compared to a library of dipeptide analogues, thus validating the uniqueness of the molecular design which confers useful properties for various potential applications.
KW - conductive composites
KW - dipeptide
KW - molecular dynamics
KW - self-assembly
KW - two-step gelation
UR - http://www.scopus.com/inward/record.url?scp=85078659982&partnerID=8YFLogxK
U2 - 10.1002/adma.201906043
DO - 10.1002/adma.201906043
M3 - Article
C2 - 31984580
AN - SCOPUS:85078659982
SN - 0935-9648
VL - 32
JO - Advanced Materials
JF - Advanced Materials
IS - 9
M1 - 1906043
ER -