Ureolysis and MICP with model and native bacteria: Implications for treatment strategies

Daniella Gat, Michael Tsesarsky, Amir Wahanon, Zeev Ronen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

11 Scopus citations

Abstract

We present results from experiments of microbially induced calcite precipitation (MICP) in co-cultures of model bacteria and results of experiments studying the ureolytic potential of native bacterial extracts. The model co-cultures experiments were designed to study possible interactions associated with introduction of exogenous ureolytic bacteria into a native soil. It was found that in co-cultures of ureolytic bacteria, S. pasteurii, with increasing concentrations of nonureolytic bacteria, B. subtillis, the rate of ureolysis and the rate of calcite precipitation increased with the concentration of B. subtillis. We postulate that the increased rates are attributed to release of urease by S. pasteurii in response to nutrient stress conditions or predation by B. subtillis. Coastal sand was tested for ureolytic capacity and nitrification potential. It was found that urea hydrolysis rate depend on nutrient media used, and that nitrifying bacteria are present in the sampled sand. Urea concentration optimization experiment performed using the bacterial extract showed that the minimal concentration of urea (supplemented with 1 gr/liter molasses) required for efficient hydrolysis is 200 mM and that for 300 mM of urea hydrolysis rate is higher by a factor of 3. These results compare well with previously reported for the model bacteria S. pasteurii.

Original languageEnglish
Title of host publicationGeo-Congress 2014 Technical Papers
Subtitle of host publicationGeo-Characterization and Modeling for Sustainability - Proceedings of the 2014 Congress
PublisherAmerican Society of Civil Engineers (ASCE)
Pages1713-1720
Number of pages8
Edition234 GSP
ISBN (Print)9780784413272
DOIs
StatePublished - 1 Jan 2014
Event2014 Congress on Geo-Characterization and Modeling for Sustainability, Geo-Congress 2014 - Atlanta, GA, United States
Duration: 23 Feb 201426 Feb 2014

Publication series

NameGeotechnical Special Publication
Number234 GSP
ISSN (Print)0895-0563

Conference

Conference2014 Congress on Geo-Characterization and Modeling for Sustainability, Geo-Congress 2014
Country/TerritoryUnited States
CityAtlanta, GA
Period23/02/1426/02/14

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Architecture
  • Building and Construction
  • Geotechnical Engineering and Engineering Geology

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