TY - GEN
T1 - Ureolytic calcium carbonate precipitation in the presence of non-ureolytic competing bacteria
AU - Gat, D.
AU - Tsesarsky, M.
AU - Shamir, D.
PY - 2011/5/27
Y1 - 2011/5/27
N2 - Stimulation of ureolytic Microbially Induced CaCO3 Precipitation (MICP) in natural soils is likely to be affected by interactions between ureolytic and non-ureolytic bacteria. In order to study these interactions we performed a MICP experiment in artificial groundwater medium, inoculated with two model bacteria: Sporosarcina pasteurii and Bacillus subtilis, and supplemented with Nutrient Broth and 7 mM of urea. Control was inoculated with S. pasteurii. The experiment lasted 10 days, during which ammonium, dissolved calcium, pH, dissolved inorganic carbon (DIC) and optical density (OD) were measured. Results show that dissolved calcium was depleted at a higher rate in the presence of non-ureolytic bacteria, despite lower pH and CO3 2- concentration. OD measurements show higher growth rate than in control samples. We suggest that the presence of the non-ureolytic bacteria, B. subtilis, accelerates MICP process, through the addition of nucleation sites in the form of bacterial cells.
AB - Stimulation of ureolytic Microbially Induced CaCO3 Precipitation (MICP) in natural soils is likely to be affected by interactions between ureolytic and non-ureolytic bacteria. In order to study these interactions we performed a MICP experiment in artificial groundwater medium, inoculated with two model bacteria: Sporosarcina pasteurii and Bacillus subtilis, and supplemented with Nutrient Broth and 7 mM of urea. Control was inoculated with S. pasteurii. The experiment lasted 10 days, during which ammonium, dissolved calcium, pH, dissolved inorganic carbon (DIC) and optical density (OD) were measured. Results show that dissolved calcium was depleted at a higher rate in the presence of non-ureolytic bacteria, despite lower pH and CO3 2- concentration. OD measurements show higher growth rate than in control samples. We suggest that the presence of the non-ureolytic bacteria, B. subtilis, accelerates MICP process, through the addition of nucleation sites in the form of bacterial cells.
KW - Bacteria
KW - Calcium carbonate
KW - Precipitation
UR - http://www.scopus.com/inward/record.url?scp=79956358964&partnerID=8YFLogxK
U2 - 10.1061/41165(397)405
DO - 10.1061/41165(397)405
M3 - Conference contribution
AN - SCOPUS:79956358964
SN - 9780784411650
T3 - Geotechnical Special Publication
SP - 3966
EP - 3974
BT - Geo-Frontiers 2011
T2 - Geo-Frontiers 2011: Advances in Geotechnical Engineering
Y2 - 13 March 2011 through 16 March 2011
ER -