Use of Fourier transform infrared microscopy for the evaluation of drug efficiency

Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun, Mahmoud Huleihel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Fourier transform infrared (FTIR) spectroscopy has been used by chemists as a powerful tool to characterize inorganic and organic compounds. In this study, we examine the potential of FTIR microspectroscopy for early evaluation of therapy efficiency. For this purpose, we examine the effect of acyclovir (a known antiherpetic drug) on the development of herpes simplex virus type 1 (HSV-1) infection in cell culture. Also, we examine spectral changes in lymphocytes obtained from leukemia patients after appropriate chemotherapy treatment. Our results show early and significant spectral indicators for successful infection of Vero cells with HSV-1. Treatment of these infected cells with increasing doses of acyclovir reduces clearly the spectral changes caused by the infection in a correlation with inhibiting the development of the cytopathic effect (CPE) induced by this virus. Also significant and consistent spectral differences between lymphocytes from human leukemia patients compared to that from healthy persons are obtained. Treatment of these leukemia patients with appropriate drugs reduces significantly these spectral differences in a correlation with the improvement of the patient's clinical situation. It seems that FTIR spectroscopy can be used as an effective tool for early evaluation of the efficiency of drugs.

Original languageEnglish
Article number064009
JournalJournal of Biomedical Optics
Volume11
Issue number6
DOIs
StatePublished - 1 Nov 2006

Keywords

  • Acyclovir
  • Drug efficiency
  • Fourier transform infrared microspectroscopy
  • Herpes simplex virus type 1
  • Leukemia

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

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