Use of FTIR spectroscopy to distinguish between capsular types and capsular quantities in Streptococcus pneumoniae

R. K. Sahu, S. Mordechai, S. Pesakhov, R. Dagan, N. Porat

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Fourier transform infrared (FTIR) spectroscopy has shown remarkable ability in distinguishing between bacterial species and identifying bacterial colony structures, when used in tandem with methods such as cluster analysis, principal component analysis, or linear discriminant analysis. The present work was aimed to evaluate the potential of FTIR-microscopy (FTIR-MSP) to distinguish between different serotypes and capsular quantities of Streptococcus pneumoniae. In general, the results obtained have consistently proven that the spectral information at the region 900-1185 cm-1 was sufficient to distinguish between various pneumococcal serotypes. Moreover, the method was able to differentiate between S. pneumoniae phase variants on the basis of their relative carbohydrate content. The unsupervised cluster analysis of the samples showed differences, not only in the carbohydrate content, but also in the region 1350-1480 cm-1, which is dominated by absorptions due to lipids and phospholipids. This approach proved to be useful for the distinction between S. pneumoniae serotypes and between phase variants, which were shown to acquire different pathogenic capacity.

Original languageEnglish
Pages (from-to)434-442
Number of pages9
JournalBiopolymers
Volume83
Issue number4
DOIs
StatePublished - 1 Nov 2006

Keywords

  • Capsule
  • Fourier transform infrared (FTIR) microscopy
  • Phenotypic variants
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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