The method of recording annhilation delayed fluorescence in the membranes of the sarcoplasmic reticulum and in the liposomes of dimyristoyl phosphatidylcholine at 30 °C has been used to study the interaction of the triplet probes between themselves and with the quencher (ferrocene). As triplet probes the authors used: perilene, 7,12-dimethyl benzanthracene and 4-(2-anthryl)-butanoic acid. The mean decay time of the annhilation delayed fluorescence of perilene and 4-(2-anthryl)-butanoic acid in the sarcoplasmic reticulum was respectively 4·2 × 10-5 and 2·3 × 10-5 sec. The rate constants of quenching of the triplet state of perilene by ferrocene are equal to 2·1 × 107 (mm/g lipid)-1sec-1 in the sarcoplasmic reticulum and 4·5 × 107 (mm/g lipid)-1sec-1 in the liposomes. Analysis of the results indicates that the sarcoplasmic reticulum lipids in the liquid crystalline state form continuous extended portions containing over 7 × 103 lipid molecules free of serious obstacles to the diffusion of the probes.
|Number of pages||6|
|Journal||Biophysics (Russian Federation)|
|State||Published - 1 Dec 1984|
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