Using Caenorhabditis elegans to screen for tissue-specific chaperone interactions

Shiran Dror, Tomer D. Meidan, Ido Karady, Anat Ben-Zvi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Correct folding and assembly of proteins and protein complexes are essential for cellular function. Cells employ quality control pathways that correct, sequester or eliminate damaged proteins to maintain a healthy proteome, thus ensuring cellular proteostasis and preventing further protein damage. Because of redundant functions within the proteostasis network, screening for detectable phenotypes using knockdown or mutations in chaperone-encoding genes in the multicellular organism Caenorhabditis elegans results in the detection of minor or no phenotypes in most cases. We have developed a targeted screening strategy to identify chaperones required for a specific function and thus bridge the gap between phenotype and function. Specifically, we monitor novel chaperone interactions using RNAi synthetic interaction screens, knocking-down chaperone expression, one chaperone at a time, in animals carrying a mutation in a chaperone-encoding gene or over-expressing a chaperone of interest. By disrupting two chaperones that individually present no gross phenotype, we can identify chaperones that aggravate or expose a specific phenotype when both perturbed. We demonstrate that this approach can identify specific sets of chaperones that function together to modulate the folding of a protein or protein complexes associated with a given phenotype.

Original languageEnglish
Article numbere61140
Pages (from-to)1-12
Number of pages12
JournalJournal of Visualized Experiments
Issue number160
StatePublished - 1 Jan 2020


  • Biology
  • Caenorhabditis elegans
  • Chaperone
  • Genetic interactions
  • Issue 160
  • Proteostasis
  • RNAi
  • Screen
  • Temperature-sensitive

ASJC Scopus subject areas

  • Neuroscience (all)
  • Chemical Engineering (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)


Dive into the research topics of 'Using Caenorhabditis elegans to screen for tissue-specific chaperone interactions'. Together they form a unique fingerprint.

Cite this