UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: An electron paramagnetic resonance (EPR) study

R. Lubart; R. Lavie; H. Friedmann; M. Sinyakov; A. Shainberg; H. Breitbart; N. Grossman

doi:10.1117/12.405915

UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: An electron paramagnetic resonance (EPR) study

R. Lubart, R. Lavie, H. Friedmann, M. Sinyakov, A. Shainberg, H. Breitbart, N. Grossman

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.

Original language	English
Pages (from-to)	18-24
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4159
DOIs	https://doi.org/10.1117/12.405915
State	Published - 1 Dec 2000
Externally published	Yes
Event	Effects of Low-Power Light on Biological Systems V - Amsterdam, Netherlands Duration: 7 Jul 2000 → 7 Jul 2000

Keywords

EPR (electron paramagnetic resonance)
ROS (reactive oxygen species)
Visible light

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.405915

Cite this

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title = "UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: An electron paramagnetic resonance (EPR) study",

abstract = "The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.",

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UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: An electron paramagnetic resonance (EPR) study. / Lubart, R.; Lavie, R.; Friedmann, H. et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4159, 01.12.2000, p. 18-24.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures

T2 - Effects of Low-Power Light on Biological Systems V

AU - Lubart, R.

AU - Lavie, R.

AU - Friedmann, H.

AU - Sinyakov, M.

AU - Shainberg, A.

AU - Breitbart, H.

AU - Grossman, N.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.

AB - The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.

KW - EPR (electron paramagnetic resonance)

KW - ROS (reactive oxygen species)

KW - Visible light

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U2 - 10.1117/12.405915

DO - 10.1117/12.405915

M3 - Conference article

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VL - 4159

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EP - 24

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

Y2 - 7 July 2000 through 7 July 2000

ER -

UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: An electron paramagnetic resonance (EPR) study

Abstract

Keywords

ASJC Scopus subject areas

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