TY - JOUR
T1 - Vaccination of European sea bass Dicentrarchus labrax with avirulent Mycobacterium marinum (iipA::kan mutant)
AU - Ravid-Peretz, Shay
AU - Colorni, Angelo
AU - Sharon, Galit
AU - Ucko, Michal
N1 - Funding Information:
We thank Mr. Ben Tamir for his technical assistance and Mrs. Barbara Colorni (both from IOLR-NCM, Eilat, Israel) for the preparation of the histological specimens. This research was carried out at the Green-Keiser Fish Health Center, IOLR-NCM, Eilat, Israel, and supported by the Israeli Ministry of National Infrastructures (grant no. 894-0197-16 ).
Funding Information:
We thank Mr. Ben Tamir for his technical assistance and Mrs. Barbara Colorni (both from IOLR-NCM, Eilat, Israel) for the preparation of the histological specimens. This research was carried out at the Green-Keiser Fish Health Center, IOLR-NCM, Eilat, Israel, and supported by the Israeli Ministry of National Infrastructures (grant no. 894-0197-16).
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Mycobacteriosis is a chronic progressive disease affecting teleost fishes all over the world. No vaccine is commercially available against its main etiological agent, Mycobacterium marinum. The mycobacterial gene responsible for invasion and intracellular persistence, iipA, is known to modulate M. marinum pathology. The innate and adaptive immune responses in sea bass (Dicentrarchus labrax) vaccinated with M. marinum iipA::kan mutant with (and without) the use of adjuvant, with (and without) a booster vaccination were monitored. The adjuvanted vaccine induced enhanced immune responses. TNF-α transcription levels were extremely high in spleen of the fish vaccinated with the addition of adjuvant in both fish vaccinated once and twice, followed by an IgM response highly specific for M. marinum. Also, histologically, granulomas started appearing in spleen and head-kidney tissues (but with no visible bacteria) within a month after vaccination, mainly with the adjuvanted vaccine. This was followed by reduction in pathology, as demonstrated by the lower number of granulomas (with visible bacteria), indicating that even heat-killed bacteria were able to elicit granulomatous formations. Adhesion of the internal organs and moderate pigmentation were observed in the perivisceral adipose tissue of nearly all vaccinated fish. Although the adjuvanted heat-killed avirulent iipA::kan mutant clearly induced a strong humoral and adaptive immune response, the booster treatment did not seem to have produced a significantly higher degree of protection from the disease compared to fish that received a single vaccination.
AB - Mycobacteriosis is a chronic progressive disease affecting teleost fishes all over the world. No vaccine is commercially available against its main etiological agent, Mycobacterium marinum. The mycobacterial gene responsible for invasion and intracellular persistence, iipA, is known to modulate M. marinum pathology. The innate and adaptive immune responses in sea bass (Dicentrarchus labrax) vaccinated with M. marinum iipA::kan mutant with (and without) the use of adjuvant, with (and without) a booster vaccination were monitored. The adjuvanted vaccine induced enhanced immune responses. TNF-α transcription levels were extremely high in spleen of the fish vaccinated with the addition of adjuvant in both fish vaccinated once and twice, followed by an IgM response highly specific for M. marinum. Also, histologically, granulomas started appearing in spleen and head-kidney tissues (but with no visible bacteria) within a month after vaccination, mainly with the adjuvanted vaccine. This was followed by reduction in pathology, as demonstrated by the lower number of granulomas (with visible bacteria), indicating that even heat-killed bacteria were able to elicit granulomatous formations. Adhesion of the internal organs and moderate pigmentation were observed in the perivisceral adipose tissue of nearly all vaccinated fish. Although the adjuvanted heat-killed avirulent iipA::kan mutant clearly induced a strong humoral and adaptive immune response, the booster treatment did not seem to have produced a significantly higher degree of protection from the disease compared to fish that received a single vaccination.
KW - Adjuvant
KW - Dicentrarchus labrax
KW - Mycobacteriosis
KW - Mycobacterium marinum
KW - Vaccine
KW - iipA mutant
UR - http://www.scopus.com/inward/record.url?scp=85065341588&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2019.04.057
DO - 10.1016/j.fsi.2019.04.057
M3 - Article
C2 - 31039442
AN - SCOPUS:85065341588
SN - 1050-4648
VL - 90
SP - 317
EP - 327
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -