TY - JOUR
T1 - Vacuolar processing enzyme activates programmed cell death in the apical meristem inducing loss of apical dominance
AU - Teper-Bamnolker, Paula
AU - Buskila, Yossi
AU - Belausov, Eduard
AU - Wolf, Dalia
AU - Doron-Faigenboim, Adi
AU - Ben-Dor, Shifra
AU - Van der Hoorn, Renier A.L.
AU - Lers, Amnon
AU - Eshel, Dani
N1 - Publisher Copyright:
© 2017 John Wiley & Sons Ltd
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The potato (Solanum tuberosum L.) tuber is a swollen underground stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (S. tuberosum vacuolar processing enzyme [StVPE]) in the tuber apical meristem (TAM). Vacuolar processing enzyme activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 (mVPE) protein exhibits specific activity for caspase 1, but not caspase 3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNA interference resulted in reduced stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to green fluorescent protein showed enhanced stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and stem branching.
AB - The potato (Solanum tuberosum L.) tuber is a swollen underground stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (S. tuberosum vacuolar processing enzyme [StVPE]) in the tuber apical meristem (TAM). Vacuolar processing enzyme activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 (mVPE) protein exhibits specific activity for caspase 1, but not caspase 3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNA interference resulted in reduced stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to green fluorescent protein showed enhanced stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and stem branching.
KW - apical dominance (AD)
KW - caspase
KW - Solanum tuberosum (potato)
KW - stem branching
KW - tuber apical meristem (TAM)
KW - vacuolar processing enzyme (VPE)
UR - http://www.scopus.com/inward/record.url?scp=85029421854&partnerID=8YFLogxK
U2 - 10.1111/pce.13044
DO - 10.1111/pce.13044
M3 - Article
C2 - 28755442
AN - SCOPUS:85029421854
SN - 0140-7791
VL - 40
SP - 2381
EP - 2392
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 10
ER -