Validation aspect in multicomponent fraction from Gokshur (Tribulus terrestris Linn.) by RP-HPLC method

Renuka, V. J. Shukla, P. K. Prajapati

Research output: Contribution to journalArticlepeer-review


Context: Analysis of botanicals is a great challenge because they are highly complex mixtures of compounds covering a broad range of substance classes and exhibit natural variability. These complex mixtures behave as matrix and may have an impact over the response factor of target analyte. Aim: In the present study, we have concentrated on detailed description of matrix effects in different Botanical reference material (BRM) samples of Tribulus terrestris in a view of liquid chromatography. Material method: As per the objective of work a HPLC method was developed by using phenomenex RPC18 column (250 x4. 6mm, 5μm), with mobile phase of Acetonitrile: Water (90:10 v/v) at the flow rate 0. 5ml/min with 20μl injection volume and UV detection at 203 nm. Results: The retention time for Diosgenin was found to be 9. 8 min. The peak obtained was well resolved by this particular method, Hence this method was selected for the study. The validation was carried out in different matrix environment of Gokshur (Tribulus terrestris Linn.) taking Diosgenin as target moiety. Conclusion: The results confirm that there is practically an interference of matrix over the response factor of target analyte. All the findings for Gokshur show that, for plant samples little purification is required. Although analysis can be carried out without purification but results will not be much accurate reliable and repetitive. Since matrix effects may exert a negative effect on important method performance parameters, they have to be tested for and evaluated during method development/validation.

Original languageEnglish
Pages (from-to)1286-1293
Number of pages8
JournalInternational Journal of PharmTech Research
Issue number3
StatePublished - 1 Jul 2013
Externally publishedYes


  • HPLC
  • Matrix effect
  • Multiple components
  • Validation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science


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