Validation of Lung EpiCheck, a novel methylation-based blood assay, for the detection of lung cancer in European and Chinese high-risk individuals

Mina Gaga, Joanna Chorostowska-Wynimko, Ildikó Horváth, Martin C. Tammemagi, David Shitrit, Vered H. Eisenberg, Hao Liang, David Stav, Dan Levy Faber, Maarten Jansen, Yael Raviv, Vasileios Panagoulias, Piotr Rudzinski, Gabriel Izbicki, Ohad Ronen, Adiv Goldhaber, Rawia Moalem, Nadir Arber, Ilana Haas, Qinghua Zhou

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Aim: Lung cancer screening reduces mortality. We aim to validate the performance of Lung EpiCheck, a six-marker panel methylation-based plasma test, in the detection of lung cancer in European and Chinese samples. Methods: A case-control European training set (n=102 lung cancer cases, n=265 controls) was used to define the panel and algorithm. Two cut-offs were selected, low cut-off (LCO) for high sensitivity and high cut-off (HCO) for high specificity. The performance was validated in case-control European and Chinese validation sets (cases/controls 179/137 and 30/15, respectively). Results: The European and Chinese validation sets achieved AUCs of 0.882 and 0.899, respectively. The sensitivities/specificities with LCO were 87.2%/64.2% and 76.7%/93.3%, and with HCO they were 74.3%/90.5% and 56.7%/100.0%, respectively. Stage I nonsmall cell lung cancer (NSCLC) sensitivity in European and Chinese samples with LCO was 78.4% and 70.0% and with HCO was 62.2% and 30.0%, respectively. Small cell lung cancer (SCLC) was represented only in the European set and sensitivities with LCO and HCO were 100.0% and 93.3%, respectively. In multivariable analyses of the European validation set, the assay's ability to predict lung cancer was independent of established risk factors (age, smoking, COPD), and overall AUC was 0.942. Conclusions: Lung EpiCheck demonstrated strong performance in lung cancer prediction in case-control European and Chinese samples, detecting high proportions of early-stage NSCLC and SCLC and significantly improving predictive accuracy when added to established risk factors. Prospective studies are required to confirm these findings. Utilising such a simple and inexpensive blood test has the potential to improve compliance and broaden access to screening for at-risk populations.

Original languageEnglish
Article number02682
JournalEuropean Respiratory Journal
Volume57
Issue number1
DOIs
StatePublished - 1 Jan 2021

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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