Vasopressin elevation of Na+/H+ exchange is inhibited by genistein in human blood platelets

Orit Aharonovits; Moriah Zik; Avinoam A. Livne; Yosef Granot

doi:10.1016/0005-2736(92)90390-8

Vasopressin elevation of Na⁺/H⁺ exchange is inhibited by genistein in human blood platelets

Orit Aharonovits, Moriah Zik, Avinoam A. Livne, Yosef Granot

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The regulation of intracellular Na⁺ and pH_i in human blood platelets is known to be controlled by the function of the Na⁺/H⁺ exchanger. The phosphorylation state of the Na⁺/H⁺ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V₁ receptor, activates the Na⁺/H⁺ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na⁺/H⁺ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na⁺/H⁺ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pH_i in human blood platelets.

Original language	English
Pages (from-to)	181-186
Number of pages	6
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1112
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(92)90390-8
State	Published - 9 Dec 1992

Keywords

(Human)
Blood platelet
Genistein
Phorbol ester
Protein kinase C
Sodium ion-proton exchange
Staurosporine
Tyrosine phosphorylation
Vasopressin

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/0005-2736(92)90390-8

Cite this

@article{06ea0f89bae1464caca419c20bc8a488,

title = "Vasopressin elevation of Na+/H+ exchange is inhibited by genistein in human blood platelets",

abstract = "The regulation of intracellular Na+ and pHi in human blood platelets is known to be controlled by the function of the Na+/H+ exchanger. The phosphorylation state of the Na+/H+ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V1 receptor, activates the Na+/H+ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na+/H+ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na+/H+ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pHi in human blood platelets.",

keywords = "(Human), Blood platelet, Genistein, Phorbol ester, Protein kinase C, Sodium ion-proton exchange, Staurosporine, Tyrosine phosphorylation, Vasopressin",

author = "Orit Aharonovits and Moriah Zik and Livne, {Avinoam A.} and Yosef Granot",

note = "Funding Information: The study was carried out at the Amelia (Mimi) Rose Laboratory for Cellular Signal Transduction, at the Department of Life Sciences, Ben-Gurion University of the Negev. We thank the Oelbaum family of Toronto, Canada, the Israeli Fund for Research and Development for financial support and Ora Cohen-Cummings for editing and wping this manuscript.",

year = "1992",

month = dec,

day = "9",

doi = "10.1016/0005-2736(92)90390-8",

language = "English",

volume = "1112",

pages = "181--186",

journal = "Biochimica et Biophysica Acta - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier B.V.",

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T1 - Vasopressin elevation of Na+/H+ exchange is inhibited by genistein in human blood platelets

AU - Aharonovits, Orit

AU - Zik, Moriah

AU - Livne, Avinoam A.

AU - Granot, Yosef

N1 - Funding Information: The study was carried out at the Amelia (Mimi) Rose Laboratory for Cellular Signal Transduction, at the Department of Life Sciences, Ben-Gurion University of the Negev. We thank the Oelbaum family of Toronto, Canada, the Israeli Fund for Research and Development for financial support and Ora Cohen-Cummings for editing and wping this manuscript.

PY - 1992/12/9

Y1 - 1992/12/9

N2 - The regulation of intracellular Na+ and pHi in human blood platelets is known to be controlled by the function of the Na+/H+ exchanger. The phosphorylation state of the Na+/H+ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V1 receptor, activates the Na+/H+ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na+/H+ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na+/H+ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pHi in human blood platelets.

AB - The regulation of intracellular Na+ and pHi in human blood platelets is known to be controlled by the function of the Na+/H+ exchanger. The phosphorylation state of the Na+/H+ exchanger which determines the exchanger activity in human blood platelets is regulated by the activities of protein kinases and protein phosphatases. Observations in this study indicate that arginine vasopressin (AVP) that interacts with a V1 receptor, activates the Na+/H+ exchange in human blood platelets through a genistein-inhibited mechanism. The AVP-activated Na+/H+ exchange is probably not regulated by protein kinase C (PKC), since this activation is not inhibited by staurosporine. The multiple ways in which platelet Na+/H+ exchange can be modulated may indicate the critical role played by this exchanger in the homeostasis control of pHi in human blood platelets.

KW - (Human)

KW - Blood platelet

KW - Genistein

KW - Phorbol ester

KW - Protein kinase C

KW - Sodium ion-proton exchange

KW - Staurosporine

KW - Tyrosine phosphorylation

KW - Vasopressin

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DO - 10.1016/0005-2736(92)90390-8

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