Verticalization of bacterial biofilms

Farzan Beroz, Jing Yan, Yigal Meir, Benedikt Sabass, Howard A. Stone, Bonnie L. Bassler, Ned S. Wingreen

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Biofilms are communities of bacteria adhered to surfaces. Recently, biofilms of rod-shaped bacteria were observed at single-cell resolution and shown to develop from a disordered, two-dimensional layer of founder cells into a three-dimensional structure with a vertically aligned core. Here, we elucidate the physical mechanism underpinning this transition using a combination of agent-based and continuum modelling. We find that verticalization proceeds through a series of localized mechanical instabilities on the cellular scale. For short cells, these instabilities are primarily triggered by cell division, whereas long cells are more likely to be peeled off the surface by nearby vertical cells, creating an ‘inverse domino effect’. The interplay between cell growth and cell verticalization gives rise to an exotic mechanical state in which the effective surface pressure becomes constant throughout the growing core of the biofilm surface layer. This dynamical isobaricity determines the expansion speed of a biofilm cluster and thereby governs how cells access the third dimension. In particular, theory predicts that a longer average cell length yields more rapidly expanding, flatter biofilms. We experimentally show that such changes in biofilm development occur by exploiting chemicals that modulate cell length.

Original languageEnglish
Pages (from-to)954-960
Number of pages7
JournalNature Physics
Volume14
Issue number9
DOIs
StatePublished - 1 Sep 2018

ASJC Scopus subject areas

  • Physics and Astronomy (all)

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