Vibrio cholerae detection: Traditional assays, novel diagnostic techniques and biosensors

Francesca Cecchini, Luka Fajs, Serge Cosnier, Robert S. Marks

Research output: Contribution to journalReview articlepeer-review

23 Scopus citations

Abstract

At present cholera remains a major public health problem. It is an acute worldwide diarrheal disease caused by the bacterium Vibrio cholerae. It affects in particular developing countries where people get infected by ingesting contaminated water and food. In this review, both internationally recognized conventional methods and new technologies in development for V. cholerae detection are reviewed. Currently, culture methods are the "gold standard" in cholera detection. Molecular techniques have a better sensitivity, but they require specific and expensive equipment. There is a need for fast, reliable, easy to use, sensitive, specific systems to use in the field during outbreaks as well as in the laboratory. Several prototype devices have been developed, but their sensitivity and specificity are low. To overcome these problems biosensors could become an attractive and efficient alternative, but they still have to be subjected to some modifications and improvements for their use in the field.

Original languageEnglish
Pages (from-to)199-209
Number of pages11
JournalTrAC - Trends in Analytical Chemistry
Volume79
DOIs
StatePublished - 1 May 2016

Keywords

  • Biosensor
  • Cholera antibodies
  • Cholera toxin
  • Molecular diagnostic
  • Rapid detection tests
  • Vibrio cholerae

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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