Viscosity and binder composition effects on tyrosinase-based carbon paste electrode for detection of phenol and catechol

Kim R. Rogers; James Y. Becker; James Cembrano; Sung H. Chough

doi:10.1016/S0039-9140(01)00376-9

Viscosity and binder composition effects on tyrosinase-based carbon paste electrode for detection of phenol and catechol

Kim R. Rogers, James Y. Becker, James Cembrano, Sung H. Chough

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The systematic study of the effect of binder viscosity on the sensitivity of a tyrosinase-based carbon paste electrode (CPE) biosensor for phenol and catechol is reported. Silicon oil binders with similar (polydimethylsiloxane) chemical composition were used to represent a wide range of viscosities (10-60 000 mPa s^-1 at 25°C) while minimizing polarity effects. The highest response for both phenol and catechol was achieved using a silicon oil binder of intermediate viscosity (100 mPa s^-1). The binder viscosity showed no appreciable effect on the direct oxidation of phenol and catechol using a plain CPE, suggesting the involvement of diffusion kinetics in the binder matrix for the enzyme-based CPE. The effect of the relative binder concentration in the carbon paste was measured over the range of 30-70%. Optimal results were obtained using 40% silicon oil. For comparison of the viscosity effects observed with the carbon paste electrode (CPE) containing silicon oil, other low and high viscosity mineral oils and paraffin waxes were also examined.

Original language	English
Pages (from-to)	1059-1065
Number of pages	7
Journal	Talanta
Volume	54
Issue number	6
DOIs	https://doi.org/10.1016/S0039-9140(01)00376-9
State	Published - 6 Jul 2001

Keywords

Biosensor
Carbon paste electrode
Phenol
Tyrosinase
Viscosity

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/S0039-9140(01)00376-9

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title = "Viscosity and binder composition effects on tyrosinase-based carbon paste electrode for detection of phenol and catechol",

abstract = "The systematic study of the effect of binder viscosity on the sensitivity of a tyrosinase-based carbon paste electrode (CPE) biosensor for phenol and catechol is reported. Silicon oil binders with similar (polydimethylsiloxane) chemical composition were used to represent a wide range of viscosities (10-60 000 mPa s-1 at 25°C) while minimizing polarity effects. The highest response for both phenol and catechol was achieved using a silicon oil binder of intermediate viscosity (100 mPa s-1). The binder viscosity showed no appreciable effect on the direct oxidation of phenol and catechol using a plain CPE, suggesting the involvement of diffusion kinetics in the binder matrix for the enzyme-based CPE. The effect of the relative binder concentration in the carbon paste was measured over the range of 30-70%. Optimal results were obtained using 40% silicon oil. For comparison of the viscosity effects observed with the carbon paste electrode (CPE) containing silicon oil, other low and high viscosity mineral oils and paraffin waxes were also examined.",

keywords = "Biosensor, Carbon paste electrode, Phenol, Tyrosinase, Viscosity",

author = "Rogers, {Kim R.} and Becker, {James Y.} and James Cembrano and Chough, {Sung H.}",

note = "Funding Information: The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research. It has been subject to the EPA's peer and administrative review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use. J.Y. Becker gratefully acknowledges support from the EPA through the National Research Council Senior Fellowship Awards Program. ",

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AU - Cembrano, James

AU - Chough, Sung H.

N1 - Funding Information: The US Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research. It has been subject to the EPA's peer and administrative review and has been approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use. J.Y. Becker gratefully acknowledges support from the EPA through the National Research Council Senior Fellowship Awards Program.

PY - 2001/7/6

Y1 - 2001/7/6

N2 - The systematic study of the effect of binder viscosity on the sensitivity of a tyrosinase-based carbon paste electrode (CPE) biosensor for phenol and catechol is reported. Silicon oil binders with similar (polydimethylsiloxane) chemical composition were used to represent a wide range of viscosities (10-60 000 mPa s-1 at 25°C) while minimizing polarity effects. The highest response for both phenol and catechol was achieved using a silicon oil binder of intermediate viscosity (100 mPa s-1). The binder viscosity showed no appreciable effect on the direct oxidation of phenol and catechol using a plain CPE, suggesting the involvement of diffusion kinetics in the binder matrix for the enzyme-based CPE. The effect of the relative binder concentration in the carbon paste was measured over the range of 30-70%. Optimal results were obtained using 40% silicon oil. For comparison of the viscosity effects observed with the carbon paste electrode (CPE) containing silicon oil, other low and high viscosity mineral oils and paraffin waxes were also examined.

AB - The systematic study of the effect of binder viscosity on the sensitivity of a tyrosinase-based carbon paste electrode (CPE) biosensor for phenol and catechol is reported. Silicon oil binders with similar (polydimethylsiloxane) chemical composition were used to represent a wide range of viscosities (10-60 000 mPa s-1 at 25°C) while minimizing polarity effects. The highest response for both phenol and catechol was achieved using a silicon oil binder of intermediate viscosity (100 mPa s-1). The binder viscosity showed no appreciable effect on the direct oxidation of phenol and catechol using a plain CPE, suggesting the involvement of diffusion kinetics in the binder matrix for the enzyme-based CPE. The effect of the relative binder concentration in the carbon paste was measured over the range of 30-70%. Optimal results were obtained using 40% silicon oil. For comparison of the viscosity effects observed with the carbon paste electrode (CPE) containing silicon oil, other low and high viscosity mineral oils and paraffin waxes were also examined.

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KW - Carbon paste electrode

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KW - Viscosity

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Viscosity and binder composition effects on tyrosinase-based carbon paste electrode for detection of phenol and catechol

Abstract

Keywords

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