Visual, rapid, and cost-effective BK virus detection system for renal transplanted patients using gold nanoparticle coupled loop-mediated isothermal amplification (nanoLAMP)

Sunil Kumar, Srishty Raman, Kishore Sesham, Abhishek Gupta, Raj Kanwar Yadav, Asit Ranjan Mridha, Subhash Chandra Yadav

Research output: Contribution to journalArticlepeer-review

Abstract

A substantial percentage of kidney transplant recipients show transplant failure due to BK virus-induced nephropathy. This can be clinically controlled by the rapid and timely detection of BK virus infection in immune-compromised patients. We report a rapid (two hours from sample collection, processing, and detection), cost-effective (< 2$), highly sensitive and BKV-specific nanoLAMP (loop-mediated isothermal amplification) diagnostic methodology using novel primers and gold nanoparticles complex-based visual detection. The standardized nanoLAMP showed an analytical sensitivity of 25 copies/µl and did not cross-react with closely related JC and SV40 viruses. This nanoLAMP showed diagnostic sensitivity and specificity as 91% and 96%, respectively, taking 50 BK virus-negative (confirmed by qPCR from the plasma of healthy donors) and 57 positive BKV patient samples (confirmed by clinical parameters and qPCR assay). This simple two-step, low-cost, and quick (1–2 h/test) detection would be advantageous over the currently used diagnostic methodology. It may change the paradigm for polyomavirus infection-based failure of renal transplant.

Original languageEnglish
Article number114889
JournalJournal of Virological Methods
Volume325
DOIs
StatePublished - 1 Apr 2024
Externally publishedYes

Keywords

  • BK virus
  • Cost-effective
  • Detection
  • Nano-LAMP
  • Renal transplantation
  • Visual test

ASJC Scopus subject areas

  • Virology

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